Bohnert J L, Horbett T A, Ratner B D, Royce F H
Department of Chemical Engineering, University of Washington, Seattle 98195.
Invest Ophthalmol Vis Sci. 1988 Mar;29(3):362-73.
A series of polymers and copolymers of 2-hydroxyethyl methacrylate (HEMA) and methyl methacrylate (MMA) were synthesized in order to find surfaces that would adsorb minimal amounts of protein. The adsorption of albumin, lysozyme and immunoglobulin G from a three-way mixture of these proteins in isotonic buffered saline to the polymers was measured using 125I-labeled proteins. Apparently high protein uptake on copolymers rich in HEMA was found to be due to sorption of unbound 125I by the polymers. 125I sorption by the polymers was minimized by dialysis of the protein solution to remove unbound 125I iodide and inclusion of 0.01 M sodium iodide to block uptake of residual 125I iodide. Using these improved protocols, minimal total protein uptake was observed on copolymers containing 50% or more HEMA. The majority of adsorbed protein on all p(MMA-HEMA) polymers was albumin. Total protein uptake was greatest on pMMA. Commercial contact lenses composed of copolymers of HEMA and N-vinyl pyrrolidone (NVP) or acrylamide (AAm) adsorbed small amounts of all proteins whereas copolymers of methacrylic acid (MAAc) and HEMA adsorbed much larger quantities of lysozyme. These results indicate that protein uptake by contact lens materials varies greatly with polymer composition. Artifactually high "adsorption" can occur if precautions are not taken to prevent uptake of unbound 125I.
合成了一系列甲基丙烯酸2-羟乙酯（HEMA）和甲基丙烯酸甲酯（MMA）的聚合物及共聚物，以寻找能吸附最少蛋白质的表面。使用125I标记的蛋白质，测定了在等渗缓冲盐水中这三种蛋白质的三元混合物对聚合物的白蛋白、溶菌酶和免疫球蛋白G的吸附情况。结果发现，富含HEMA的共聚物上明显较高的蛋白质摄取量是由于聚合物对未结合的125I的吸附。通过对蛋白质溶液进行透析以去除未结合的125I碘化物，并加入0.01 M碘化钠以阻止残留125I碘化物的摄取，聚合物对125I的吸附被降至最低。使用这些改进的方案，在含有50%或更多HEMA的共聚物上观察到总蛋白质摄取量最少。所有p（MMA-HEMA）聚合物上吸附的蛋白质大部分是白蛋白。pMMA上的总蛋白质摄取量最大。由HEMA与N-乙烯基吡咯烷酮（NVP）或丙烯酰胺（AAm）的共聚物组成的商业隐形眼镜吸附了少量的所有蛋白质，而甲基丙烯酸（MAAc）与HEMA的共聚物吸附了大量的溶菌酶。这些结果表明，隐形眼镜材料对蛋白质的摄取量随聚合物组成的不同而有很大差异。如果不采取预防措施防止未结合的125I的摄取，可能会出现人为的高“吸附”现象。
