Weisman L S, Ballou C E
Department of Biochemistry, University of California, Berkeley 94720.
J Bacteriol. 1988 Mar;170(3):1393-5. doi: 10.1128/jb.170.3.1393-1395.1988.
Mycobacterium smegmatis cells produce [3H]methanol when incubated with [methyl-3H]methionine. The methanol is derived from S-adenosylmethionine rather than methyltetrahydrofolate. M. smegmatis cells carboxymethylate several proteins, and some of the methanol probably results from their demethylation, but most of the methanol may come from an unidentified component with a high gel mobility. Although methanol in the medium reached 19 microM, it was not incorporated into the methylated mannose polysaccharide, a lipid carrier in this organism.
耻垢分枝杆菌细胞在与[甲基-³H]甲硫氨酸一起孵育时会产生[³H]甲醇。甲醇来源于S-腺苷甲硫氨酸而非甲基四氢叶酸。耻垢分枝杆菌细胞会使几种蛋白质发生羧甲基化，部分甲醇可能是其去甲基化的产物，但大部分甲醇可能来自一种凝胶迁移率高且身份不明的成分。尽管培养基中的甲醇浓度达到了19微摩尔，但它并未掺入该生物体中的脂质载体——甲基化甘露糖多糖中。
