Department of Genetics, Stanford University, Stanford, CA, USA.
Cancer Biology Program, Stanford University, Stanford, CA, USA.
Nat Methods. 2021 Feb;18(2):133-143. doi: 10.1038/s41592-020-01010-5. Epub 2021 Jan 11.
Many biological processes are executed and regulated through the molecular interactions of proteins and nucleic acids. Proximity labeling (PL) is a technology for tagging the endogenous interaction partners of specific protein 'baits', via genetic fusion to promiscuous enzymes that catalyze the generation of diffusible reactive species in living cells. Tagged molecules that interact with baits can then be enriched and identified by mass spectrometry or nucleic acid sequencing. Here we review the development of PL technologies and highlight studies that have applied PL to the discovery and analysis of molecular interactions. In particular, we focus on the use of PL for mapping protein-protein, protein-RNA and protein-DNA interactions in living cells and organisms.
许多生物过程是通过蛋白质和核酸的分子相互作用来执行和调节的。邻近标记 (PL) 是一种通过遗传融合到广谱酶来标记特定蛋白质“诱饵”的内源性相互作用伙伴的技术，这些酶可以在活细胞中催化可扩散反应性物质的产生。然后可以通过质谱或核酸测序来富集和鉴定与诱饵相互作用的标记分子。在这里，我们回顾了 PL 技术的发展，并强调了将 PL 应用于分子相互作用的发现和分析的研究。特别是，我们专注于使用 PL 来绘制活细胞和生物体中的蛋白质-蛋白质、蛋白质-RNA 和蛋白质-DNA 相互作用图谱。
