Department of Chemical Physiology & Biochemistry, Oregon Health & Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA.
Chem Commun (Camb). 2021 Feb 7;57(11):1344-1347. doi: 10.1039/d0cc07042d. Epub 2021 Jan 12.
The RNA aptamer Broccoli accepts 2'fluorinated (2'F) pyrimidine nucleotide incorporation without perturbation of structure or fluorescence in the presence of potassium and DFHBI. However, the modification decreases Broccoli's apparent affinity for K >30-fold. A chimera of Broccoli RNAs with mixed chemistries displays linear fluorescent gain spanning physiological K concentrations, yielding an effective RNA-based fluorescent K sensor.
Broccoli 是一种 RNA 适体，在钾离子和 DFHBI 的存在下，能够接受 2'位氟代（2'F）嘧啶核苷酸的掺入，而不会影响其结构或荧光。然而，这种修饰会使 Broccoli 对钾离子的表观亲和力降低 30 多倍。具有混合化学性质的 Broccoli RNA 嵌合体在生理范围内的钾离子浓度下表现出线性荧光增益，从而产生一种有效的基于 RNA 的荧光钾离子传感器。
