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火眼灯鱼(Characiformes: Characidae)的胚胎发育。

Embryonic development of the fire-eye-tetra (Characiformes: Characidae).

机构信息

Research Group of Studies on the Reproduction of Amazon fish (GERPA/LANEC), Faculdade de Biologia (FACBIO), University of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil.

Aquam Research Group, Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.

出版信息

Zygote. 2021 Jun;29(3):194-198. doi: 10.1017/S0967199420000647. Epub 2021 Jan 12.

Abstract

This study describes the embryonic development of Moenkhausia oligolepis in laboratory conditions. After fertilization, the embryos were collected every 10 min up to 2 h, then every 20 min up to 4 h, and afterwards every 30 min until hatching. The fertilized eggs of M. oligolepis measured approximately 0.85 ± 0.5 mm and had an adhesive surface. Embryonic development lasted 14 h at 25ºC through the zygote, cleavage, blastula, gastrula, neurula, and segmentation phases. Hatching occurred in embryos around the 30-somites stage. The present results contribute only the second description of embryonic development to a species from the Moenkhausia genus, being also the first for this species. Such data are of paramount importance considering the current conflicting state of this genus phylogenetic classification and may help taxonomic studies. Understanding the biology of a species that is easily managed in laboratory conditions and has an ornamental appeal may assist studies in its reproduction to both supply the aquarium market and help the species conservation in nature. Moreover, these data enable the use of M. oligolepis as a model species in biotechnological applications, such as the germ cell transplantation approach.

摘要

本研究描述了实验室条件下莫氏脂鲤胚胎的发育过程。受精后,每隔 10 分钟收集一次胚胎,持续 2 小时,然后每隔 20 分钟收集一次,持续 4 小时,之后每隔 30 分钟收集一次,直到孵化。莫氏脂鲤的受精卵大小约为 0.85 ± 0.5 毫米,具有粘性表面。在 25°C 的条件下,胚胎发育经过合子、卵裂、囊胚、原肠胚、神经胚和分节期,持续了 14 小时。孵化发生在大约 30 体节阶段的胚胎中。本研究结果仅对莫氏脂鲤属的第二个物种进行了胚胎发育描述,也是该物种的首次描述。考虑到该属系统发育分类目前存在冲突的状态,这些数据非常重要,可能有助于分类学研究。了解在实验室条件下容易管理且具有观赏价值的物种的生物学特性,可能有助于对其进行繁殖研究,以供应水族馆市场,并帮助该物种在自然界中的保护。此外,这些数据还使得莫氏脂鲤能够作为生物技术应用的模型物种,如生殖细胞移植方法。

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