Bao Lei, Chau Cecilia S, Lei Zhengdeng, Hu Hong, Chan Angelina G, Amber Kyle T, Maienschein-Cline Mark, Tsoukas Maria M
Department of Dermatology, UIC-Dermatology, RM 338, MC624, 808 S. Wood Street, Chicago, IL, 60612, USA.
Sequencing Core, Genome Research Division, Research Resources Center, Chicago, USA.
Arch Dermatol Res. 2021 Dec;313(10):837-846. doi: 10.1007/s00403-020-02176-w. Epub 2021 Jan 12.
IL-4 plays an important role in the pathogenesis of atopic dermatitis (AD). Previously we showed that the expression of genes in chemotaxis, angiogenesis, inflammation and barrier functions is dysregulated in IL-4 transgenic (Tg) mice, a well-characterized AD mouse model. In this study, we aim to study differential expression of microRNAs in IL-4 Tg mice. As compared with wild-type mice, we found that 10 and 79 microRNAs are dysregulated in the skin of IL-4 mice before and after the onset of skin lesions, respectively. Bioinformatic analysis and previous reports show that these dysregulated microRNAs may be involved in the NF-κB, TLRs, IL-4/IL-13, MAPK and other pathways. We also found that miR-139-5p and miR-196b-3p are significantly up-regulated in the peripheral blood of IL-4 Tg mice. Taken together, our data have identified many dysregulated microRNAs in IL-4 Tg mice, which may play important roles in AD pathogenesis and pathophysiology.
白细胞介素-4（IL-4）在特应性皮炎（AD）的发病机制中起重要作用。此前我们发现，在白细胞介素-4转基因（Tg）小鼠（一种特征明确的AD小鼠模型）中，趋化、血管生成、炎症和屏障功能相关基因的表达失调。在本研究中，我们旨在研究白细胞介素-4转基因小鼠中微小RNA的差异表达。与野生型小鼠相比，我们发现分别在皮肤病变发生之前和之后，白细胞介素-4转基因小鼠皮肤中有10个和79个微小RNA表达失调。生物信息学分析和既往报道表明，这些表达失调的微小RNA可能参与核因子κB（NF-κB）、Toll样受体（TLRs）、白细胞介素-4/白细胞介素-13（IL-4/IL-13）、丝裂原活化蛋白激酶（MAPK）等信号通路。我们还发现，在白细胞介素-4转基因小鼠的外周血中，miR-139-5p和miR-196b-3p显著上调。综上所述，我们的数据鉴定出了白细胞介素-4转基因小鼠中许多表达失调的微小RNA，它们可能在AD的发病机制和病理生理学中发挥重要作用。
