Department of Pulmonary Medicine, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, China.
Department of Pulmonary Medicine, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, China.
Life Sci. 2021 Mar 1;268:119022. doi: 10.1016/j.lfs.2021.119022. Epub 2021 Jan 9.
This study aimed to characterize the functions of pseudogene-derived long non-coding RNA (lncRNA) FAM207BP in lung adenocarcinoma (LUAD).
Through the Cancer Genome Atlas (TCGA)-Genotype Tissue Expression (GTEx) database, FAM207BP expression was detected in LUAD and normal tissues. Overall survival (OS) and disease-free survival (DFS) analysis was presented using log-rank test or univariate Cox regression analysis. The relationships between FAM207BP expression and clinical features were analyzed. FAM207BP expression was validated in LUAD tissues and cells using RT-qPCR. Cell viability of LUAD cells was evaluated after silencing or overexpressing FAM207BP. Furthermore, migrated and invasive abilities were examined by Transwell and scratch assays. The correlation between FAM207BP expression and the immune infiltration levels was analyzed. Gene Set Enrichment Analysis (GSEA) was performed for high- and low-expression of FAM207BP using C2 collection in the Molecular Signatures Database (MSigDB) database.
FAM207BP expression was distinctly higher in LUAD than normal tissues. Patients with its high expression indicated worse OS and DFS time. FAM207BP expression was significantly related to gender. RT-qPCR results confirmed that FAM207BP was significantly highly expressed in LUAD tissues and cells. Knockdown of FAM207BP distinctly suppressed cellular viability, migration and invasion for LUAD cells. Also, its expression was negatively related to B cell infiltration levels. GSEA results indicated that high FAM207BP expression was involved in regulation of gene expression. Its low expression was related to immune response.
Pseudogene-derived lncRNA FAM207BP could induce proliferation and migration of LUAD cells, which could act as an immune-related prognostic factor.
本研究旨在探讨假基因衍生的长链非编码 RNA（lncRNA） FAM207BP 在肺腺癌（LUAD）中的功能。
通过癌症基因组图谱（TCGA）-基因组织表达（GTEx）数据库，检测 LUAD 和正常组织中 FAM207BP 的表达。使用对数秩检验或单因素 Cox 回归分析进行总生存（OS）和无病生存（DFS）分析。分析 FAM207BP 表达与临床特征的关系。采用 RT-qPCR 验证 FAM207BP 在 LUAD 组织和细胞中的表达。沉默或过表达 FAM207BP 后，评估 LUAD 细胞的活力。通过 Transwell 和划痕实验检测迁移和侵袭能力。分析 FAM207BP 表达与免疫浸润水平的相关性。使用分子特征数据库（MSigDB）中的 C2 集合，对 FAM207BP 高表达和低表达进行基因集富集分析（GSEA）。
FAM207BP 在 LUAD 中的表达明显高于正常组织。高表达的患者 OS 和 DFS 时间更差。FAM207BP 的表达与性别显著相关。RT-qPCR 结果证实 FAM207BP 在 LUAD 组织和细胞中显著高表达。沉默 FAM207BP 明显抑制 LUAD 细胞的细胞活力、迁移和侵袭。此外，其表达与 B 细胞浸润水平呈负相关。GSEA 结果表明，高 FAM207BP 表达参与基因表达的调控。低表达与免疫反应有关。
假基因衍生的 lncRNA FAM207BP 可诱导 LUAD 细胞的增殖和迁移，可作为与免疫相关的预后因素。
