Department of Oncology and Hematology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.
Histol Histopathol. 2024 May;39(5):595-602. doi: 10.14670/HH-18-642. Epub 2023 Jun 26.
Long non-coding RNAs (lncRNAs) have been used in the study of tumor biomarkers in recent years. However, the prognostic role of lncRNA LINC00924 (LINC00924) in lung adenocarcinoma (LUAD) has not yet been concluded. Therefore, this study investigates the prognostic value of LINC00924 in LUAD and its regulatory effect on tumor progression.
The LUAD tissues and adjacent normal tissues of 128 subjects were extracted, and the expressions of LINC00924 and miR-196a-5p in tissues and cells were detected by RT-qPCR. The prognostic value of LINC00924 in LUAD patients was obtained by Kaplan-Meier analysis and multivariate Cox regression test. The cell counting kit-8 (CCK-8) and Transwell assay were used to detect the effect of overexpression LINC00924 on LUAD cells.
In LUAD tissues and cells, LINC00924 expression was down-regulated and miR-196a-5p expression was up-regulated compared with the normal control group. High expression of LINC00924 inhibited the proliferation level, migration ability and invasion situation of LUAD cells, which was more conducive to the survival and prognosis of LUAD patients. Bioinformatics studies indicated that overexpression of LINC00924 inhibited the development of LUAD by targeting miR-196a-5p, while miR-196a-5p mimic effectively weakened the inhibition.
LINC00924 sponges of miR-196a-5p may be considered as a potential prognostic biomarker for LUAD.
近年来，长链非编码 RNA（lncRNA）已被用于肿瘤标志物的研究。然而，lncRNA LINC00924（LINC00924）在肺腺癌（LUAD）中的预后作用尚未得出结论。因此，本研究探讨了 LINC00924 在 LUAD 中的预后价值及其对肿瘤进展的调控作用。
提取 128 例患者的 LUAD 组织及相邻正常组织，采用 RT-qPCR 检测组织及细胞中 LINC00924 和 miR-196a-5p 的表达。通过 Kaplan-Meier 分析和多因素 Cox 回归检验获得 LINC00924 在 LUAD 患者中的预后价值。采用细胞计数试剂盒-8（CCK-8）和 Transwell 实验检测过表达 LINC00924 对 LUAD 细胞的影响。
与正常对照组相比，LUAD 组织和细胞中 LINC00924 表达下调，miR-196a-5p 表达上调。LINC00924 高表达抑制 LUAD 细胞的增殖水平、迁移能力和侵袭情况，更有利于 LUAD 患者的生存和预后。生物信息学研究表明，过表达 LINC00924 通过靶向 miR-196a-5p 抑制 LUAD 的发生发展，而 miR-196a-5p 模拟物可有效减弱抑制作用。
LINC00924 可能通过海绵吸附 miR-196a-5p 被视为 LUAD 的潜在预后生物标志物。
