Department of Molecular and Cellular Biology, Faculty of Pharmacy, Medical University of Wroclaw, Poland.
Łukasiewicz Research Network - PORT Polish Center for Technology Development, Bioimaging Laboratory, Wroclaw, Poland.
Bioelectrochemistry. 2021 Apr;138:107728. doi: 10.1016/j.bioelechem.2020.107728. Epub 2020 Dec 29.
Melanoma is considered the most aggressive type of skin cancer, still without effective treatment. Thus, alternative therapeutic methods are still in demand, and electroporation-supported photodynamic therapy (EP-PDT) of cancer cells seems a promising approach. New developments in EP-PDT aim at enhanced tumor selectivity and biocompatibility by applying a second-generation photosensitizer, i.e., Chlorin e6 (Ce6). We have verified the improved photodynamic effect of Ce6 on skin cancer melanoma (Me45) cells and control (CHO-K1) cells. In this study, we applied 1 or 5 pulses of 10 ms duration and assessed the EP-PDT effect with a variety of tests, such as singlet oxygen scavenger (ABMDMA) photooxidation, oxidoreductive potential measurements, kinetic measurements with fluorescent microscopy, photosensitizer uptake studies, lipid peroxidation level, and actin fibers organization. The optimization of photosensitizer uptake as a function of temperature was also performed. Our results indicated efficient Ce6 delivery into Me45 cells and good photodynamic efficiency enhanced by the electroporation of cancer cells.
黑色素瘤被认为是最具侵袭性的皮肤癌，目前仍没有有效的治疗方法。因此，仍然需要替代的治疗方法，而电穿孔辅助光动力疗法（EP-PDT）治疗癌细胞似乎是一种很有前途的方法。第二代光敏剂，即 Chlorin e6（Ce6）的应用，旨在提高 EP-PDT 的肿瘤选择性和生物相容性。我们已经验证了 Ce6 对皮肤癌黑色素瘤（Me45）细胞和对照（CHO-K1）细胞的光动力效应的改善。在这项研究中，我们施加了 1 或 5 个 10ms 持续时间的脉冲，并通过各种测试评估了 EP-PDT 效应，例如单线态氧清除剂（ABMDMA）光氧化、氧化还原电位测量、荧光显微镜的动力学测量、光敏剂摄取研究、脂质过氧化水平和肌动蛋白纤维组织。还优化了作为温度函数的光敏剂摄取。我们的结果表明，Ce6 能够有效地递送到 Me45 细胞中，并且通过电穿孔增强了癌细胞的光动力效率。
