Matsuda Shofu, Hitsuji Airi, Nakanishi Takuya, Zhang Hong, Tanaka Akane, Matsuda Hiroshi, Osaka Tetsuya
Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, 3-8-1 Harumi-cho, Fuchu, Tokyo 183-0057, Japan.
ACS Biomater Sci Eng. 2015 Aug 10;1(8):632-638. doi: 10.1021/acsbiomaterials.5b00009. Epub 2015 Jul 9.
Nanoparticle uptake and cell death following addition of magnetite nanoparticles (MNPs) with a diameter of ∼10 nm were evaluated in three histological types of human mesothelioma cells, NCI-H28 (epithelioid), NCI-H2052 (sarcomatoid), and MSTO-211H (biphasic) cells, and human breast cancer MCF-7 cells. Dose-dependent cell death was observed in MSTO-211H cells but not in MCF-7 cells, although cellular uptake of MNPs was observed in both cell types. Mesothelioma NCI-H28 and NCI-H2052 cells showed behavior more similar to that of breast cancer MCF-7 cells than that of mesothelioma MSTO-211H cells. DNA fragmentation and microarray analyses suggested that MNPs induced transforming growth factor β2 related apoptosis in MSTO-211H cells. On the other hand, the viability of human mesothelioma cells containing MNPs with a diameter of ∼40 nm was investigated after exposure to an alternating magnetic field. Temperature increase under the alternating magnetic field and high rates of cell death were observed in all three histological types of human mesothelioma.
在三种组织学类型的人恶性间皮瘤细胞（NCI - H28（上皮样）、NCI - H2052（肉瘤样）和MSTO - 211H（双向性）细胞）以及人乳腺癌MCF - 7细胞中，评估了添加直径约为10 nm的磁铁矿纳米颗粒（MNP）后的纳米颗粒摄取和细胞死亡情况。尽管在两种细胞类型中均观察到MNP的细胞摄取，但在MSTO - 211H细胞中观察到剂量依赖性细胞死亡，而在MCF - 7细胞中未观察到。恶性间皮瘤NCI - H28和NCI - H2052细胞表现出的行为与乳腺癌MCF - 7细胞更相似，而不是与恶性间皮瘤MSTO - 211H细胞相似。DNA片段化和微阵列分析表明，MNP在MSTO - 211H细胞中诱导了与转化生长因子β2相关的细胞凋亡。另一方面，在暴露于交变磁场后，研究了含有直径约40 nm的MNP的人恶性间皮瘤细胞的活力。在所有三种组织学类型的人恶性间皮瘤中均观察到交变磁场下的温度升高和高细胞死亡率。
