Iacovita Cristian, Fizeșan Ionel, Pop Anca, Scorus Lavinia, Dudric Roxana, Stiufiuc Gabriela, Vedeanu Nicoleta, Tetean Romulus, Loghin Felicia, Stiufiuc Rares, Lucaciu Constantin Mihai
Department of Pharmaceutical Physics-Biophysics, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Pasteur 6, 400349 Cluj-Napoca, Romania.
Department of Toxicology, Faculty of Pharmacy, "Iuliu Hațieganu" University of Medicine and Pharmacy, Pasteur, 6A, 400349 Cluj-Napoca, Romania.
Pharmaceutics. 2020 May 6;12(5):424. doi: 10.3390/pharmaceutics12050424.
We report the synthesis of magnetite nanoparticles (IOMNPs) using the polyol method performed at elevated temperature (300 °C) and high pressure. The ferromagnetic polyhedral IOMNPs exhibited high saturation magnetizations at room temperature (83 emu/g) and a maximum specific absorption rate (SAR) of 2400 W/g in water. The uniform dispersion of IOMNPs in solid matrix led to a monotonous increase of SAR maximum (3600 W/g) as the concentration decreased. Cytotoxicity studies on two cell lines (cancer and normal) using Alamar Blues and Neutral Red assays revealed insignificant toxicity of the IOMNPs on the cells up to a concentration of 1000 μg/mL. The cells internalized the IOMNPs inside lysosomes in a dose-dependent manner, with higher amounts of IOMNPs in cancer cells. Intracellular hyperthermia experiments revealed a significant increase in the macroscopic temperatures of the IOMNPs loaded cell suspensions, which depend on the amount of internalized IOMNPs and the alternating magnetic field amplitude. The cancer cells were found to be more sensitive to the intracellular hyperthermia compared to the normal ones. For both cell lines, cells heated at the same macroscopic temperature presented lower viability at higher amplitudes of the alternating magnetic field, indicating the occurrence of mechanical or nanoscale heating effects.
我们报道了采用多元醇法在高温（300°C）和高压下合成磁铁矿纳米颗粒（IOMNPs）。铁磁多面体IOMNPs在室温下表现出高饱和磁化强度（83 emu/g），在水中的最大比吸收率（SAR）为2400 W/g。IOMNPs在固体基质中的均匀分散导致随着浓度降低，SAR最大值单调增加（3600 W/g）。使用阿拉玛蓝和中性红试验对两种细胞系（癌细胞和正常细胞）进行的细胞毒性研究表明，在浓度高达1000μg/mL时，IOMNPs对细胞的毒性不显著。细胞以剂量依赖的方式将IOMNPs内化到溶酶体中，癌细胞中的IOMNPs含量更高。细胞内热疗实验表明，加载IOMNPs的细胞悬液的宏观温度显著升高，这取决于内化IOMNPs的量和交变磁场幅度。发现癌细胞比正常细胞对细胞内热疗更敏感。对于两种细胞系，在相同宏观温度下加热的细胞在交变磁场较高幅度时活力较低，表明发生了机械或纳米级加热效应。
