Anderson Jennifer L, Niedert Elizabeth E, Patnaik Sourav S, Tang Renxiang, Holloway Riley L, Osteguin Vangelina, Finol Ender A, Goergen Craig J
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
Department of Mechanical Engineering, University of Texas, San Antonio, TX 78249, USA.
J Clin Med. 2021 Jan 9;10(2):219. doi: 10.3390/jcm10020219.
Abdominal aortic aneurysms (AAAs) are a local dilation of the aorta and are associated with significant mortality due to rupture and treatment complications. There is a need for less invasive treatments to prevent aneurysm growth and rupture. In this study, we used two experimental murine models to evaluate the potential of pentagalloyl glucose (PGG), which is a polyphenolic tannin that binds to and crosslinks elastin and collagen, to preserve aortic compliance. Animals underwent surgical aortic injury and received 0.3% PGG or saline treatment on the adventitial surface of the infrarenal aorta. Seventeen mice underwent topical elastase injury, and 14 mice underwent topical calcium chloride injury. We collected high-frequency ultrasound images before surgery and at 3-4 timepoints after. There was no difference in the in vivo effective maximum diameter due to PGG treatment for either model. However, the CaCl model had significantly higher Green-Lagrange circumferential cyclic strain in PGG-treated animals ( < 0.05). While ex vivo pressure-inflation testing showed no difference between groups in either model, histology revealed reduced calcium deposits in the PGG treatment group with the CaCl model. These findings highlight the continued need for improved understanding of PGG's effects on the extracellular matrix and suggest that PGG may reduce arterial calcium accumulation.
腹主动脉瘤（AAAs）是主动脉的局部扩张，与因破裂和治疗并发症导致的显著死亡率相关。需要采用侵入性较小的治疗方法来预防动脉瘤的生长和破裂。在本研究中，我们使用两种实验性小鼠模型来评估五倍没食子酰葡萄糖（PGG）的潜力，PGG是一种能与弹性蛋白和胶原蛋白结合并交联的多酚单宁，用于维持主动脉顺应性。动物接受主动脉手术损伤，并在肾下腹主动脉外膜表面接受0.3% PGG或生理盐水治疗。17只小鼠接受局部弹性蛋白酶损伤，14只小鼠接受局部氯化钙损伤。我们在手术前和术后3 - 4个时间点采集高频超声图像。两种模型中，PGG治疗组的体内有效最大直径均无差异。然而，在氯化钙模型中，PGG治疗的动物的格林 - 拉格朗日周向循环应变显著更高（<0.05）。虽然体外压力膨胀测试显示两种模型中各治疗组之间没有差异，但组织学显示氯化钙模型中PGG治疗组的钙沉积减少。这些发现凸显了持续深入了解PGG对细胞外基质作用的必要性，并表明PGG可能减少动脉钙积累。
