School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, China.
China Animal Disease Control Centre, Beijing, China.
SLAS Technol. 2021 Aug;26(4):377-383. doi: 10.1177/2472630320981935. Epub 2021 Jan 12.
O157:H7 ( O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of O157:H7. The detection sensitivity of the strip is 10 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.
O157:H7（O157：H7）是一种危险的食源性致病菌，主要存在于牛肉、牛奶、水果及其制品中，对人类健康造成危害，甚至可能导致死亡。因此，对食品中 O157：H7 的检测显得尤为重要。本文报告了一种基于聚集诱导发光（AIE）材料标记抗原的侧向流动免疫测定条（LFIS）作为荧光探针，用于快速检测 O157：H7。该条的检测灵敏度为 10 CFU/mL，比胶体金测试条高 10 倍。该方法具有良好的特异性和稳定性，可成功检测 25 g 或 25 mL 牛肉、果冻和牛奶中的约 250 CFU 的 O157：H7。AIE-LFIS 可能在快速检测食品病原体的监测方面具有重要价值。
