College of Food Science, Southwest University, Chongqing, 400715, PR China; Chongqing Key Laboratory of Chinese Medicine & Health Science, Chongqing Academy of Chinese Materia Medica, Chongqing, 400065, PR China.
College of Food Science, Southwest University, Chongqing, 400715, PR China; College of Environment and Resources, Chongqing Technology and Business University, Chongqing, 400067, PR China.
Carbohydr Polym. 2021 Mar 1;255:117368. doi: 10.1016/j.carbpol.2020.117368. Epub 2020 Nov 5.
Effect and working mechanism of temperature and pH on encapsulation and release of β-carotene from octenylsuccinated-oat-β-glucan-micelles (OSβG-Ms) were investigated. The stability and solubility of β-carotene, and changes in surface hydrophilicity, core hydrophobicity, and size of β-carotene-loaded-OSβG-Ms were determined. When exposed to temperature (25-45 °C) and pH (4.5-8.5), β-carotene solubilization changed in parabolic manners. Size and absolute zeta-potential of β-carotene-loaded-OSβG-Ms decreased with temperature, while they gave parabolic changing patterns with pH. Those results were ascribed to their hydrophilicity, hydrophobicity, and core/shell compactness via regulating molecule mobility, orientation, and interactions by temperature/pH. The higher temperature concluded with higher β-carotene release, while a U-shaped release profile was observed with pH. Besides its diffusion, erosion-induced shrinking and collapsing of OSβG-Ms favored β-carotene release at pH 1.2-4.5, which was replaced by swelling-induced structural-relaxation at pH 6.8-8.5. The results were favourable in controlling the behavior of β-carotene-loaded-OSβG-Ms by selectively applying environmental parameters.
研究了温度和 pH 值对辛烯基琥珀酸燕麦β-葡聚糖胶束（OSβG-Ms）包封和释放 β-胡萝卜素的影响及作用机制。测定了 β-胡萝卜素的稳定性和溶解度，以及负载 β-胡萝卜素的 OSβG-Ms 的表面亲水性、内核疏水性和粒径的变化。当暴露于温度（25-45°C）和 pH（4.5-8.5）时，β-胡萝卜素的溶解呈抛物线变化。β-胡萝卜素负载的 OSβG-Ms 的粒径和绝对 zeta 电位随温度的升高而降低，而随 pH 值的升高呈抛物线变化。这些结果归因于温度/pH 通过调节分子的流动性、取向和相互作用对其亲水性、疏水性和核/壳紧密性的影响。较高的温度导致 β-胡萝卜素释放增加，而 pH 值呈 U 型释放曲线。除了扩散之外，在 pH 值为 1.2-4.5 时，OSβG-Ms 的侵蚀诱导收缩和崩塌有利于 β-胡萝卜素的释放，而在 pH 值为 6.8-8.5 时，溶胀诱导的结构松弛则取代了这种释放方式。这些结果有利于通过选择性应用环境参数来控制负载β-胡萝卜素的 OSβG-Ms 的行为。
