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心脏骤停相关垂体损伤的评估：一种实验性啮齿动物模型。

The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model.

机构信息

The Feinstein Institutes for Medical Research, Northwell Health, 350 Community Dr., Manhasset, NY, 11030, USA.

Elmezzi Graduate School of Molecular Medicine at Northwell Health, Manhasset, NY, USA.

出版信息

Sci Rep. 2021 Jan 12;11(1):629. doi: 10.1038/s41598-020-79780-3.

DOI:10.1038/s41598-020-79780-3
PMID:33436714
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7804952/
Abstract

The pituitary gland plays an important endocrinal role, however its damage after cardiac arrest (CA) has not been well elucidated. The aim of this study was to determine a pituitary gland damage induced by CA. Rats were subjected to 10-min asphyxia and cardiopulmonary resuscitation (CPR). Immunohistochemistry and ELISA assays were used to evaluate the pituitary damage and endocrine function. Samples were collected at pre-CA, and 30 and 120 min after cardio pulmonary resuscitation. Triphenyltetrazolium chloride (TTC) staining demonstrated the expansion of the pituitary damage over time. There was phenotypic validity between the pars distalis and nervosa. Both CT-proAVP (pars nervosa hormone) and GH/IGF-1 (pars distalis hormone) decreased over time, and a different expression pattern corresponding to the damaged areas was noted (CT-proAVP, 30.2 ± 6.2, 31.5 ± 5.9, and 16.3 ± 7.6 pg/mg protein, p < 0.01; GH/IGF-1, 2.63 ± 0.61, 0.62 ± 0.36, and 2.01 ± 0.41 ng/mg protein, p < 0.01 respectively). Similarly, the expression pattern between these hormones in the end-organ systems showed phenotypic validity. Plasma CT-proAVP (r = 0.771, p = 0.025) and IGF-1 (r = -0.775, p = 0.024) demonstrated a strong correlation with TTC staining area. Our data suggested that CA induces pathological and functional damage to the pituitary gland.

摘要

垂体在内分泌中发挥着重要作用，但心脏骤停（CA）后其损伤尚未得到充分阐明。本研究旨在确定 CA 引起的垂体损伤。大鼠接受 10 分钟窒息和心肺复苏（CPR）。免疫组织化学和 ELISA 检测用于评估垂体损伤和内分泌功能。标本采集于 CA 前、CPR 后 30 分钟和 120 分钟。三苯基四氮唑（TTC）染色显示垂体损伤随时间扩展。神经部和远侧部之间存在表型一致性。CT-proAVP（神经部激素）和 GH/IGF-1（远侧部激素）随时间降低，并且观察到与损伤区域相对应的不同表达模式（CT-proAVP，30.2±6.2、31.5±5.9 和 16.3±7.6 pg/mg 蛋白，p<0.01；GH/IGF-1，2.63±0.61、0.62±0.36 和 2.01±0.41 ng/mg 蛋白，p<0.01）。同样，这些激素在终末器官系统中的表达模式也具有表型一致性。血浆 CT-proAVP（r=0.771，p=0.025）和 IGF-1（r=-0.775，p=0.024）与 TTC 染色面积具有很强的相关性。我们的数据表明，CA 会导致垂体的病理性和功能性损伤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14dd/7804952/12b0b1f8ebbb/41598_2020_79780_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14dd/7804952/3c9f591a89c8/41598_2020_79780_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14dd/7804952/12b0b1f8ebbb/41598_2020_79780_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14dd/7804952/3c9f591a89c8/41598_2020_79780_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14dd/7804952/651babcc710d/41598_2020_79780_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14dd/7804952/dcd25132db6c/41598_2020_79780_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14dd/7804952/102f17613eb1/41598_2020_79780_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14dd/7804952/e38daade7493/41598_2020_79780_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14dd/7804952/12b0b1f8ebbb/41598_2020_79780_Fig6_HTML.jpg

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