Faculty of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa, 240-8501, Japan.
Kanagawa Institute of Industrial Science and Technology, 3-2-1 Sakado Takatsu-ku, Kawasaki, Kanagawa, 213-0012, Japan.
Sci Rep. 2021 Jan 12;11(1):624. doi: 10.1038/s41598-020-79722-z.
Hair regenerative medicine has emerged as a promising approach for the treatment of severe hair loss. Recent advances in three-dimensional tissue engineering, such as formation of hair follicle germs (HFGs), have considerably improved hair regeneration after transplantation in animal models. Here, we proposed an approach for fabricating HFGs containing vascular endothelial cells. Epithelial, dermal papilla, and vascular endothelial cells initially formed a single aggregate, which subsequently became a dumbbell-shaped HFG, wherein the vascular endothelial cells localized in the region of dermal papilla cells. The HFGs containing vascular endothelial cells exhibited higher expression of hair morphogenesis-related genes in vitro, along with higher levels of hair shaft regeneration upon transplantation to the dorsal side of nude mice, than those without vascular endothelial cells. The generated hair follicles represented functional characteristics, such as piloerection, as well as morphological characteristics comparable to those of natural hair shafts. This approach may provide a promising strategy for fabricating tissue grafts with higher hair inductivity for hair regenerative medicine.
毛发再生医学已成为治疗严重脱发的一种有前途的方法。最近在三维组织工程方面的进展，如毛囊胚芽（HFGs）的形成，极大地改善了动物模型中移植后的毛发再生。在这里，我们提出了一种制造含有血管内皮细胞的 HFGs 的方法。上皮细胞、真皮乳头和血管内皮细胞最初形成一个单一的聚集物，随后形成一个哑铃形的 HFG，其中血管内皮细胞定位于真皮乳头细胞区域。与不含血管内皮细胞的 HFGs 相比，含有血管内皮细胞的 HFGs 在体外表现出更高的毛发形态发生相关基因表达水平，并且在移植到裸鼠背部后，毛发轴的再生水平更高。生成的毛囊具有毛发竖起以及与天然毛发轴相当的形态特征等功能特性。这种方法可能为毛发再生医学提供一种制造具有更高毛发诱导能力的组织移植物的有前途的策略。
