Department of Oral Rehabilitation, Oral Implantology, Fukuoka Dental College, Fukuoka, Japan.
Department of Restorative Dentistry, Oregon Health & Science University School of Dentistry, Portland, OR, USA.
Sci Rep. 2021 Jan 12;11(1):205. doi: 10.1038/s41598-020-80316-y.
Bone marrow ablation prompts transient bone formation in nearly the entire medullary cavity before marrow regeneration occurs. Here, we establish a procedure to direct bone formation in a desired particular site within the medullary cavity for support of biomedical devices. Local intramedullary injury was performed in the tibiae of rats and parathyroid hormone (PTH), alendronate, or saline was administered. Newly generated bone in the medulla was assessed by micro-CT and histology. To evaluate the function of newly generated bone, animals received intramedullary injury in tibiae followed by daily PTH. At day-14, implants were placed in the endocortical bone and the bone response to the implants was assessed. The fate of newly generated bone was compared with and without implants. We found that neither intramedullary injury nor medication alone resulted in bone formation. However, when combined, substantial bone was generated locally inside the diaphyseal medulla. Newly formed bone disappeared without implant placement but was retained with implants. Bone was especially retained around and between the implants. This study found that local bone marrow disruption followed by PTH or alendronate generated substantial cancellous bone locally in the diaphyseal medulla. This approach offers promise as a tissue engineering tool in medicine and dentistry.
骨髓消融会在骨髓再生发生之前，在几乎整个骨髓腔内引发短暂的骨形成。在这里，我们建立了一种在骨髓腔内的特定部位引导骨形成的方法，以支持生物医学设备。在大鼠的胫骨中进行局部髓内损伤，并给予甲状旁腺激素（PTH）、阿伦膦酸盐或生理盐水。通过 micro-CT 和组织学评估骨髓中新生的骨。为了评估新生成的骨的功能，动物在胫骨中接受髓内损伤，然后每天给予 PTH。在第 14 天，将植入物放置在内皮质骨中，并评估植入物对骨的反应。比较了有无植入物时新生成骨的命运。我们发现，单独进行髓内损伤或药物治疗都不会导致骨形成。然而，当两者结合时，大量的骨会在骨干髓腔内局部生成。没有植入物时，新形成的骨会消失，但有植入物时会保留下来。骨特别保留在植入物周围和之间。这项研究发现，局部骨髓破坏后使用 PTH 或阿伦膦酸盐可在骨干髓腔内局部产生大量松质骨。这种方法有望成为医学和牙科领域的组织工程工具。
