具有静磁场的聚多巴胺涂层通过上调BMP-Smads信号通路促进人骨源间充质干细胞在三维打印多孔钛支架上的成骨分化。
Polydopamine coating with static magnetic field promotes the osteogenic differentiation of human bone-derived mesenchymal stem cells on three-dimensional printed porous titanium scaffolds by upregulation of the BMP-Smads signaling pathway.
作者信息
Kong Lingpeng, Han Yong, Lu Qingsen, Zhou Dongsheng, Wang Bomin, Wang Dawei, Zhang Wupeng, Xiang Hao, Li Mingzhen, Wang Fu
机构信息
Department of Orthopaedics, Shandong Provincial Hospital Affiliated to Shandong First Medical University No. 324, Jingwu Road, Jinan 250021, Shandong, P. R. China.
出版信息
Am J Transl Res. 2020 Dec 15;12(12):7812-7825. eCollection 2020.
Bone regeneration has always been a hot topic for orthopedic surgeons. The role of polydopamine coating in promoting bone regeneration has attracted much attention. Static magnetic field (SMF) is considered an effective and noninvasive treatment for enhancing bone regeneration. However, the effect of polydopamine combined with SMF on bone regeneration on scaffolds is not clear. The aim of this study was to investigate the effects and potential mechanism of polydopamine coating combined with SMF on bone regeneration in three-dimensional printed scaffolds. The polydopamine coating (pTi group) was applied onto porous Ti6Al4V scaffolds (Ti group). Surface characterization was performed by scanning electron microscopy. The 100 mT SMF environment (pTi-SMF group) was established to enhance osteogenic differentiation of human bone-derived mesenchymal stem cells (hBMSCs) on polydopamine coating scaffolds. The cell viability and proliferation were significantly enhanced in the SMF environment (pTi-SMF Ti: P=0.005). Improved morphology (pTi-SMF pTi: P=0.024, pTi-SMF Ti: P=0.001) and adhesion (Ti: x̅±s=1.585±0.324; pTi: x̅±s=2.164±0.314; pTi-SMF: x̅±s=4.634±0.247, P<0.001) of hBMSCs were observed in the pTi-SMF group. The high expression of osteogenesis-related RNA and protein (ALP: Ti, x̅±s=1.249±0.218; pTi, x̅±s=2.503±0.209; pTi-SMF, x̅±s=2.810±0.246. OCN: Ti, x̅±s=1.483±0.304; pTi, x̅±s=3.636±0.322; pTi-SMF, x̅±s=4.641±0.278. Runx2: Ti, x̅±s=1.372±0.227; pTi, x̅±s=3.054±0.229; pTi-SMF, x̅±s=3.914±0.253) was found in the pTi-SMF group (pTi-SMF Ti: P<0.001). Proteomics was applied to explore the osteogenic mechanism of polydopamine coating combined with SMF. A total of 147 different proteins were identified between the pTi-SMF and Ti group. The osteogenic effect might be associated with the BMP-Smads signaling pathway (pTi-SMF Ti: BMPR1A, P=0.001; BMPR2, P<0.001; Smad4, P=0.001; Smad1/5/8, P=0.008). In conclusion, the osteogenic differentiation of hBMSCs on polydopamine coating scaffolds could be enhanced by SMF stimulation by upregulation of the BMP-Smads signaling pathway.
骨再生一直是骨科医生关注的热点话题。聚多巴胺涂层在促进骨再生方面的作用备受关注。静磁场（SMF）被认为是促进骨再生的一种有效且无创的治疗方法。然而，聚多巴胺与静磁场联合作用于支架上骨再生的效果尚不清楚。本研究旨在探讨聚多巴胺涂层联合静磁场对三维打印支架骨再生的影响及潜在机制。将聚多巴胺涂层（pTi组）应用于多孔Ti6Al4V支架（Ti组）。通过扫描电子显微镜进行表面表征。建立100 mT的静磁场环境（pTi-SMF组）以增强人骨源性间充质干细胞（hBMSCs）在聚多巴胺涂层支架上的成骨分化。在静磁场环境下，细胞活力和增殖显著增强（pTi-SMF Ti：P = 0.005）。在pTi-SMF组观察到hBMSCs的形态（pTi-SMF pTi：P = 0.024，pTi-SMF Ti：P = 0.001）和黏附（Ti：x̅±s = 1.585±0.324；pTi：x̅±s = 2.164±0.314；pTi-SMF：x̅±s = 4.634±0.247，P < 0.001）得到改善。在pTi-SMF组发现成骨相关RNA和蛋白质高表达（碱性磷酸酶：Ti，x̅±s = 1.249±0.218；pTi，x̅±s = 2.503±0.209；pTi-SMF，x̅±s = 2.810±0.246。骨钙素：Ti，x̅±s = 1.483±0.304；pTi，x̅±s = 3.636±0.322；pTi-SMF，x̅±s = 4.641±0.278。Runx2：Ti，x̅±s = 1.372±0.227；pTi，x̅±s = 3.054±0.229；pTi-SMF，x̅±s = 3.914±0.253）（pTi-SMF Ti：P < 0.001）。应用蛋白质组学探索聚多巴胺涂层联合静磁场的成骨机制。在pTi-SMF组和Ti组之间共鉴定出147种不同的蛋白质。成骨作用可能与骨形态发生蛋白-Smads信号通路有关（pTi-SMF Ti：骨形态发生蛋白受体1A，P = 0.001；骨形态发生蛋白受体2，P < 0.001；Smad4，P = 0.001；Smad1/5/8，P = 0.008）。总之，通过上调骨形态发生蛋白-Smads信号通路，静磁场刺激可增强hBMSCs在聚多巴胺涂层支架上的成骨分化。
Zotero 插件