Feng Bobo, Sundin Elin, Lincoln Per, Mårtensson Anna K F
Department of Chemistry and Chemical Engineering, Kemigården 4, SE-412 96 Gothenburg, Sweden.
Phys Chem Chem Phys. 2021 Jan 28;23(3):2238-2244. doi: 10.1039/d0cp00845a.
The enantiomers of a novel mononuclear ruthenium(ii) complex [Ru(phen)2bidppz]2+ with an elongated dppz moiety were synthesized. Surprisingly, the complex showed no DNA intercalating capability in an aqueous environment. However, by the addition of water-miscible polyethylene glycol ether PEG-400, self-aggregation of the hydrophobic ruthenium(ii) complexes was counter-acted, thus strongly promoting the DNA intercalation binding mode. This mild alteration of the environment surrounding the DNA polymer does not damage or alter the DNA structure but instead enables more efficient binding characterization studies of potential DNA binding drugs.
合成了一种具有延长的二吡啶并菲（dppz）部分的新型单核钌（II）配合物[Ru(phen)2bidppz]2+的对映体。令人惊讶的是，该配合物在水性环境中没有DNA嵌入能力。然而，通过加入与水混溶的聚乙二醇醚PEG - 400，疏水性钌（II）配合物的自聚集被抵消，从而强烈促进了DNA嵌入结合模式。DNA聚合物周围环境的这种轻微改变不会破坏或改变DNA结构，而是能够对潜在的DNA结合药物进行更有效的结合表征研究。
