Lai-Fook S J, Kaplowitz M R
Cardiovascular Research Institute, University of California, San Francisco.
Microvasc Res. 1988 Jan;35(1):101-8. doi: 10.1016/0026-2862(88)90053-2.
To determine the effect of systemic vascular hypertension on fluid balance in the pleural space, we studied the spontaneously hypertensive rat (SHR) and its genetic normotensive control, the Wistar-Kyoto rat (WKY). We measured arterial and venous pressures, total protein and albumin concentrations of pleural liquid and plasma, pleural space thickness, and pleural surface pressure in SHR and WKY that were matched for weight (260-300 g). Protein concentration was measured by a manual Biuret test and albumin concentration was measured by the bromcresol green colorimetric method. Pleural liquid thickness was measured in situ using light microscopy. Pleural surface pressure was assumed to equal pleural liquid pressure. In the SHR, total protein and albumin concentrations in pleural liquid were lower than in WKY, and pleural space thickness was larger in SHR than in WKY. These results are consistent with a higher capillary pressure and greater fluid filtration in SHR.
为了确定全身性血管高血压对胸腔内液体平衡的影响，我们研究了自发性高血压大鼠（SHR）及其遗传性血压正常对照——Wistar-Kyoto大鼠（WKY）。我们测量了体重匹配（260 - 300克）的SHR和WKY的动脉压和静脉压、胸腔液体和血浆中的总蛋白及白蛋白浓度、胸腔间隙厚度以及胸腔表面压力。总蛋白浓度通过手工双缩脲试验测量，白蛋白浓度通过溴甲酚绿比色法测量。使用光学显微镜原位测量胸腔液体厚度。假定胸腔表面压力等于胸腔液体压力。在SHR中，胸腔液体中的总蛋白和白蛋白浓度低于WKY，且SHR的胸腔间隙厚度大于WKY。这些结果与SHR中较高的毛细血管压力和更大的液体滤过一致。
