锌指蛋白 2 与肝癌免疫浸润的相关性：一个预后生物标志物。

Correlation of zinc finger protein 2, a prognostic biomarker, with immune infiltrates in liver cancer.

机构信息

Clinical College of Weifang Medical University, Weifang, 261053, China.

Department of oncology, Affiliated Hospital of Weifang Medical University, Weifang, 261031, China.

出版信息

Biosci Rep. 2021 Jan 29;41(1). doi: 10.1042/BSR20203115.

DOI:10.1042/BSR20203115

PMID:33439969

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7823187/

Abstract

PURPOSE

The expression and clinical value of zinc finger protein 2 gene (ZIC2) in hepatocellular carcinoma (HCC) were analyzed by mining gene information from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases.

METHODS

Gene chip data sets were retrieved from GEO and TCGA and screened for differentially expressed genes in HCC. Gene expression profile interaction analysis (GEPIA) and Kaplan-Meier curves were used to analyze the relationship between differentially expressed genes (DEGs) and survival and prognosis in patients with HCC. Moreover, the Genecards database was used to extract ZIC2-related proteins and to analyze the physiological process of protein enrichment. Furthermore, the relationships between ZIC2 gene and tumor cell immune invasion and that between immune cell infiltration and the 5-year survival rate were studied using the tumor immune evaluation resource (TIMER) database.

RESULTS

Datasets from GEO and TCGA revealed that ZIC2 was differentially expressed in HCC tissues and normal tissues (P<0.05). High ZIC2 expression was associated with overall survival (OS) and progress-free survival in HCC patients. Overall, 25 ZIC2 related proteins, including Gli3, PRKDC, and rnf180 were identified and protein enrichment analysis indicated these were associated with four types of cell components, six types of cell functions, and eight types of biological processes. ZIC2 was positively correlated with immune infiltration cells in patients with HCC, and higher expression of ZIC2 mRNA CD4+T cells is associated with a better 5-year survival.

CONCLUSION

ZIC2 gene may be used as an immune response marker in liver cancer to predict the prognosis of HCC.

摘要

目的

通过挖掘癌症基因组图谱（TCGA）和基因表达综合数据库（GEO）中的基因信息，分析锌指蛋白 2 基因（ZIC2）在肝细胞癌（HCC）中的表达及临床价值。

方法

从 GEO 和 TCGA 中检索基因芯片数据集，筛选 HCC 中差异表达的基因。采用基因表达谱交互分析（GEPIA）和 Kaplan-Meier 曲线分析差异表达基因（DEGs）与 HCC 患者生存和预后的关系。此外，利用 Genecards 数据库提取 ZIC2 相关蛋白，并对蛋白富集的生理过程进行分析。进一步利用肿瘤免疫评估资源（TIMER）数据库研究 ZIC2 基因与肿瘤细胞免疫浸润的关系，以及免疫细胞浸润与 5 年生存率的关系。

结果

GEO 和 TCGA 的数据集显示，ZIC2 在 HCC 组织和正常组织中的表达存在差异（P<0.05）。ZIC2 高表达与 HCC 患者的总生存期（OS）和无进展生存期（PFS）相关。共鉴定出 25 个 ZIC2 相关蛋白，包括 Gli3、PRKDC 和 rnf180，蛋白富集分析表明这些蛋白与四种细胞成分、六种细胞功能和八种生物过程相关。ZIC2 与 HCC 患者的免疫浸润细胞呈正相关，ZIC2mRNA 高表达的 CD4+T 细胞与更好的 5 年生存率相关。