Department of General Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL, United States.
Front Immunol. 2019 Jan 29;10:6. doi: 10.3389/fimmu.2019.00006. eCollection 2019.
Layilin (LAYN) is a critical gene that regulates T cell function. However, the correlations of LAYN to prognosis and tumor-infiltrating lymphocytes in different cancers remain unclear. LAYN expression was analyzed via the Oncomine database and Tumor Immune Estimation Resource (TIMER) site. We evaluated the influence of LAYN on clinical prognosis using Kaplan-Meier plotter, the PrognoScan database and Gene Expression Profiling Interactive Analysis (GEPIA). The correlations between LAYN and cancer immune infiltrates was investigated via TIMER. In addition, correlations between LAYN expression and gene marker sets of immune infiltrates were analyzed by TIMER and GEPIA. A cohort (GSE17536) of colorectal cancer patients showed that high LAYN expression was associated with poorer overall survival (OS), disease-specific survival (DSS), and disease-free survival (DFS). In addition, high LAYN expression was significantly correlated with poor OS and progression-free survival (PFS) in gastric cancers (OS HR = 1.97, = 3.6e-10; PFS HR = 2.12, = 2.3e-10). Moreover, LAYN significantly impacts the prognosis of diverse cancers via The Cancer Genome Atlas (TCGA). Specifically, high LAYN expression was correlated with worse OS and PFS in stage 2 to 4 but not stage 1 and stage N0 gastric cancer patients ( = 0.28, 0.34; = 0.073, 0.092). LAYN expression was positively correlated with infiltrating levels of CD4+ T and CD8+ T cells, macrophages, neutrophils, and dendritic cells (DCs) in colon adenocarcinoma (COAD) and stomach adenocarcinoma (STAD). LAYN expression showed strong correlations with diverse immune marker sets in COAD and STAD. These findings suggest that LAYN is correlated with prognosis and immune infiltrating levels of, including those of CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and DCs in multiple cancers, especially in colon and gastric cancer patients. In addition, LAYN expression potentially contributes to regulation of tumor-associated macrophages (TAMs), DCs, T cell exhaustion and Tregs in colon and gastric cancer. These findings suggest that LAYN can be used as a prognostic biomarker for determining prognosis and immune infiltration in gastric and colon cancers.
Layilin (LAYN) 是一个关键的基因，它调节 T 细胞的功能。然而，LAYN 与不同癌症的预后和肿瘤浸润淋巴细胞之间的相关性尚不清楚。通过 Oncomine 数据库和 Tumor Immune Estimation Resource (TIMER) 网站分析 LAYN 的表达。我们使用 Kaplan-Meier plotter、PrognoScan 数据库和 Gene Expression Profiling Interactive Analysis (GEPIA) 来评估 LAYN 对临床预后的影响。通过 TIMER 研究 LAYN 与癌症免疫浸润之间的相关性。此外，通过 TIMER 和 GEPIA 分析 LAYN 表达与免疫浸润基因标志物集之间的相关性。一个结直肠癌患者队列 (GSE17536) 显示，高 LAYN 表达与总生存期 (OS)、疾病特异性生存期 (DSS) 和无病生存期 (DFS) 较差相关。此外，高 LAYN 表达与胃癌患者的不良 OS 和无进展生存期 (PFS) 显著相关 (OS HR = 1.97, = 3.6e-10; PFS HR = 2.12, = 2.3e-10)。此外，LAYN 通过癌症基因组图谱 (TCGA) 显著影响多种癌症的预后。具体来说，高 LAYN 表达与 2 期至 4 期而非 1 期和 N0 期胃癌患者的 OS 和 PFS 较差相关 ( = 0.28, 0.34; = 0.073, 0.092)。在结肠腺癌 (COAD) 和胃腺癌 (STAD) 中，LAYN 表达与 CD4+ T 和 CD8+ T 细胞、巨噬细胞、中性粒细胞和树突状细胞 (DC) 的浸润水平呈正相关。LAYN 表达与 COAD 和 STAD 中的多种免疫标志物集呈强相关性。这些发现表明，LAYN 与包括 CD8+ T 细胞、CD4+ T 细胞、巨噬细胞、中性粒细胞和 DC 在内的多种癌症的预后和免疫浸润水平相关，尤其是在结肠和胃癌患者中。此外，LAYN 表达可能有助于调节结肠和胃癌中的肿瘤相关巨噬细胞 (TAMs)、DC、T 细胞耗竭和 Tregs。这些发现表明，LAYN 可作为用于确定胃和结肠癌预后和免疫浸润的预后生物标志物。
