Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Langmuir. 2021 Jan 26;37(3):1082-1088. doi: 10.1021/acs.langmuir.0c02843. Epub 2021 Jan 13.
Giant unilamellar vesicles (GUVs) adsorb to a solid surface and rupture to form a planar bilayer patch. These bilayer patches are used to investigate the properties and functions of biological membranes. Therefore, it is crucial to understand the mechanisms of GUV adsorption. In this study, we investigate the adsorption of phase-separated GUVs on glass using fluorescence microscopy. GUVs containing liquid-ordered (L) and liquid-disordered (L) phases underwent domain sorting after adsorption. The L domain in the unbound region migrated to the highly curved region near the edge of the adsorbed region. Additionally, the L phase grew linearly along the edge of the adsorbed region, creating a thin ring-like domain. After the domain sorting event, the GUV ruptured to form a planar bilayer patch with circular-patterned domains in the initially adsorbed area. We found that domain sorting was promoted by increasing the extent of GUV deformation. These results suggest that both the L and L domains are reorganized for stabilizing the curved bilayer region in adsorbed GUVs.
巨单层囊泡（GUV）会吸附在固体表面并破裂形成平面双层斑片。这些双层斑片可用于研究生物膜的性质和功能。因此，了解 GUV 吸附的机制至关重要。在这项研究中，我们使用荧光显微镜研究了相分离 GUV 在玻璃上的吸附。吸附后，含有有序相（L）和无序相（L）的 GUV 发生了域排序。未结合区域的 L 域迁移到吸附区域边缘附近的高度弯曲区域。此外，L 相沿着吸附区域的边缘线性生长，形成一个薄的环状域。在域排序事件之后，GUV 破裂形成一个平面双层斑片，在最初吸附的区域有圆形图案的域。我们发现，随着 GUV 变形程度的增加，域排序得到促进。这些结果表明，L 和 L 域都被重新组织以稳定吸附 GUV 中的弯曲双层区域。
