Roy Ekta, Patra Santanu, Madhuri Rashmi, Sharma Prashant K
Department of Applied Chemistry and ‡Functional Nanomaterials Research Laboratory, Department of Applied Physics, Indian Institute of Technology (Indian School of Mines), Dhanbad, Jharkhand 826 004, India.
Department of Applied Chemistry and Functional Nanomaterials Research Laboratory, Department of Applied Physics, Indian Institute of Technology (Indian School of Mines), Dhanbad, Jharkhand 826 004, India.
ACS Biomater Sci Eng. 2017 Sep 11;3(9):2120-2135. doi: 10.1021/acsbiomaterials.7b00089. Epub 2017 Mar 31.
Herein, we report the advanced polymer vesicle [made up of triblock polymer: poly(ethylene oxide)--poly(Cys-AuNP@FA)--poly(3-methoxypropylacrylamide] having encapsulated magnetic nanoparticle capable of targeted methotrexate delivery (having folic acid as tagging agent), photothermal therapy [anisotropic gold nanoparticle (AuNPs)] and stimuli-responsive T-imaging (as MRI contrast agent). The prepared polymersome, called as magnetopolymersome (MPS), after encapsulation of magnetic nanoparticle (Gd-doped) is not only high yield and simple in synthesis but also possess very high biocompatibility, more than 95% drug encapsulation efficiency and effective near-infrared (NIR) responsive photothermal therapy. The MPS is highly stable under normal physiological environments and other extreme end conditions (like presence of serum or Triton-X 100) and have excellent stimuli-responsive (temperature and NIR) T-contrast effect in vitro conditions (60.57 mM s). To explore the role of shape of AuNPs on the photothermal therapy and drug delivery behavior of prepared nanocarrier, herein, we have synthesized four different shapes of AuNPs, i.e., spherical, triangle, rod, and flower. It was found that nanoflower-conjugated MPS shows the most efficient NIR responsive behavior in comparison to their other colleagues, which broke the ancient myth that spherical nanoparticle are the best candidate for drug delivery process. These features make nanoflower or other anisotropic nanoparticle-based polymersome a very promising and efficient nanocarrier for drug loading, delivery, imaging, and photothermal therapy.
在此，我们报道了一种先进的聚合物囊泡[由三嵌段聚合物组成：聚（环氧乙烷）-聚（半胱氨酸-金纳米粒子@叶酸）-聚（3-甲氧基丙基丙烯酰胺）]，其包裹有能够进行甲氨蝶呤靶向递送（以叶酸作为标记剂）、光热疗法[各向异性金纳米粒子（AuNPs）]和刺激响应性T成像（作为磁共振成像造影剂）的磁性纳米粒子。制备的聚合物囊泡，称为磁聚合物囊泡（MPS），在包裹磁性纳米粒子（钆掺杂）后，不仅合成产率高且简单，还具有非常高的生物相容性、超过95%的药物包封效率以及有效的近红外（NIR）响应光热疗法。MPS在正常生理环境和其他极端条件（如存在血清或曲拉通-X 100）下高度稳定，并且在体外条件（60.57 mM s）下具有出色的刺激响应（温度和NIR）T造影效果。为了探究金纳米粒子的形状对所制备纳米载体的光热疗法和药物递送行为的作用，在此，我们合成了四种不同形状的金纳米粒子，即球形、三角形、棒状和花状。结果发现，与其他同类相比，纳米花共轭的MPS表现出最有效的近红外响应行为，这打破了球形纳米粒子是药物递送过程最佳候选者的古老神话。这些特性使基于纳米花或其他各向异性纳米粒子的聚合物囊泡成为用于药物负载、递送、成像和光热疗法的非常有前景且高效的纳米载体。
