Tian Qiaomu, Deo Michael, Rivera-Castaneda Laura, Liu Huinan
Department of Bioengineering, University of California, Riverside, California 92521, United States.
Material Science & Engineering Program, University of California, Riverside, California 92521, United States.
ACS Biomater Sci Eng. 2016 Sep 12;2(9):1559-1571. doi: 10.1021/acsbiomaterials.6b00325. Epub 2016 Aug 9.
Magnesium (Mg) is a biodegradable metallic material, which has shown great potential for medical device applications. In this study, human urothelial cells (HUCs) were cultured with Mg-based substrates to investigate their cytocompatibility for potential urological device applications. Three different culture methodologies were explored to mimic different conditions, in an attempt to establish standard methods of evaluating cytocompatibility of Mg-based biomaterials for urological device applications. Direct culture is a suitable method when it is important to evaluate direct cell attachment on the biomaterial surfaces. Direct exposure culture is a desirable method for investigating the response of well-established cells in the body with newly implanted biomaterials. The exposure culture method is appropriate for evaluating cell-biomaterial interactions in the same environment, where they are not in direct contact with each other. The results showed differences in HUC behaviors with the same Mg-based substrates when different culture methods were used. The Mg-based substrates inhibited the HUC viability with direct contact at the cell-material interface in direct culture and direct exposure culture. The faster degrading Mg alloys containing yttrium reduced HUC density in direct culture, direct exposure culture, and exposure culture. The major soluble degradation products of Mg-based materials reduced HUC density significantly when the pH increased to 8.6 and above or the Mg ion concentration reached 10 mM and above. Mg-based biomaterials, especially the slower degrading alloys such as AZ31, should be further studied to determine their potential to be used for bioresorbable urological devices.
镁（Mg）是一种可生物降解的金属材料，在医疗设备应用方面显示出巨大潜力。在本研究中，将人尿路上皮细胞（HUCs）与镁基基底进行培养，以研究其在潜在泌尿外科设备应用中的细胞相容性。探索了三种不同的培养方法来模拟不同条件，试图建立评估镁基生物材料在泌尿外科设备应用中细胞相容性的标准方法。直接培养是评估细胞在生物材料表面直接附着情况时的一种合适方法。直接暴露培养是研究体内已建立的细胞对新植入生物材料反应的理想方法。暴露培养方法适用于评估在同一环境中细胞与生物材料之间的相互作用，此时它们彼此不直接接触。结果表明，当使用不同培养方法时，相同镁基基底上的HUC行为存在差异。在直接培养和直接暴露培养中，镁基基底在细胞 - 材料界面直接接触时抑制了HUC的活力。含钇的降解速度较快的镁合金在直接培养、直接暴露培养和暴露培养中降低了HUC密度。当pH值升至8.6及以上或镁离子浓度达到10 mM及以上时，镁基材料的主要可溶性降解产物显著降低了HUC密度。应进一步研究镁基生物材料，特别是像AZ31这样降解较慢的合金，以确定其用于可生物吸收泌尿外科设备的潜力。
