Chovanes G I, McAllister J P, Lamperti A A, Salotto A G, Truex R C
Department of Neurosurgery, Temple University School of Medicine, Philadelphia, Pennsylvania.
Neurosurgery. 1988 Jan;22(1 Pt 1):86-91. doi: 10.1227/00006123-198801010-00014.
The present study was designed to determine the selected monoamine changes that occur during infantile hydrocephalus. Obstructive hydrocephalus was induced in newborn rats by injection of a suspension of kaolin into the 4th ventricle and cisterna magna. Eleven days later, experimental animals and their sham-operated littermate controls were killed and pieces of frontoparietal cortex, neostriatum, cerebellar vermis, and brain stem were processed for high performance liquid chromatography. Grossly, the lateral ventricles were extremely enlarged, the cerebral cortex was thinned, the neostriatum was compressed, and portions of the tectum and cerebellum were vacuolated. Decreases in norepinephrine (71%), dopamine (73%), and serotonin (50%) were observed in the cerebral cortex, neostriatum, and cerebellum, respectively. Brain stem norepinephrine and serotonin were increased 70% and 50%, respectively. These increases may indicate impairment of axonal transport or damage to projections from the locus ceruleus and raphe region. These preliminary results suggest that infantile hydrocephalus causes perturbations in the levels of different monoamines in several brain regions. Such changes may critically influence neuronal function and development, as well as the therapeutic management of hydrocephalus.
本研究旨在确定婴儿脑积水期间发生的选定单胺变化。通过向第四脑室和大池注射高岭土悬液，在新生大鼠中诱发梗阻性脑积水。11天后，处死实验动物及其假手术同窝对照，取额顶叶皮质、新纹状体、小脑蚓部和脑干组织进行高效液相色谱分析。大体观察可见，侧脑室极度扩大，大脑皮质变薄，新纹状体受压，顶盖和小脑部分出现空泡化。在大脑皮质、新纹状体和小脑中，去甲肾上腺素（71%）、多巴胺（73%）和血清素（50%）分别减少。脑干中的去甲肾上腺素和血清素分别增加了70%和50%。这些增加可能表明轴突运输受损或蓝斑和中缝区域投射受损。这些初步结果表明，婴儿脑积水会导致几个脑区不同单胺水平的紊乱。这种变化可能会严重影响神经元功能和发育，以及脑积水的治疗管理。
