Valvano M A, Hartstein A I, Morthland V H, Dragoon M E, Potter S A, Reynolds J W, Crosa J H
Department of Microbiology, Oregon Health Sciences University, Portland 97201.
Pediatr Infect Dis J. 1988 Feb;7(2):116-20. doi: 10.1097/00006454-198802000-00010.
We prospectively studied the course of colonization and sepsis with Staphylococcus epidermidis among 29 very low birth weight neonates undergoing prolonged umbilical catheterization. S. epidermidis bacteremia occurred in 7 patients. In 6 bacteremia was preceded by positive colonization cultures. Isolates obtained from nares, base of umbilicus, umbilical catheter entry sites, catheter tips and blood were examined for plasmid DNA profiles. In 4 patients the plasmid profiles of the catheter entry site isolates were identical with those of the blood isolates. In the other 3 bacteremic patients plasmid profiles of the catheter entry site and blood isolates were different. No correlation was observed in the plasmid DNA patterns of isolates obtained from catheter tip cultures as compared to the corresponding blood cultures. The blood isolates from bacteremic patients had different plasmid profiles.
我们前瞻性地研究了29例接受长时间脐静脉插管的极低出生体重儿表皮葡萄球菌的定植过程和败血症情况。7例患儿发生了表皮葡萄球菌菌血症。其中6例在菌血症发生前定植培养呈阳性。对从鼻腔、脐根部、脐静脉插管入口处、导管尖端和血液中分离出的菌株进行质粒DNA图谱检测。4例患儿导管入口处分离菌株的质粒图谱与血液分离菌株的相同。另外3例菌血症患儿导管入口处和血液分离菌株的质粒图谱不同。与相应血培养相比，导管尖端培养分离菌株的质粒DNA图谱未观察到相关性。菌血症患儿的血液分离菌株具有不同的质粒图谱。
