Horticultural Sciences Department, University of Florida, Gainesville, FL, 32611, USA.
Everglades Research and Education Center, Institute of Food and Agricultural Sciences/University of Florida, Belle Glade, FL, 33430, USA.
Sci Rep. 2021 Jan 13;11(1):998. doi: 10.1038/s41598-020-80050-5.
Liriomyza trifolii (Diptera: Agromyzidae) is a leafminer that causes ruinous damage to many leafy vegetables including lettuce (Lactuca sativa L.) by stippling and tunneling the leaves. In this study, a population of 125 F families was developed from the intraspecific cross of 'Valmaine' (resistant) and 'Okeechobee' (susceptible) romaine cultivars for inheritance analysis and molecular mapping of the resistance loci controlling stippling damage. The experiments were conducted in an insectarium (controlled environment). Stippling damage proved to be heritable because the broad-sense heritability (H) was 0.58. A segregation analysis suggested that a single dominant allele, Sd1 locus, controls resistance against L. trifolii. Furthermore, a quantitative trait loci (QTL) analysis identified one novel QTL, named Stippling on LG5 (qSTP5), flanked by two SNPs that were mapped to a 5.2 cM (8.5 Mb region) interval, explaining over 13% of the total phenotypic variance. Desirable allele for resistance to L. trifolii was derived from resistant cultivar Valmaine. Identification of SNPs closely linked to the QTL responsible for L. trifolii resistance should facilitate plant breeders to develop resistant romaine lettuce cultivars.
三叶草斑潜蝇（双翅目：潜蝇科）是一种潜叶蝇，通过在叶片上造成点刻和隧道，对包括生菜（生菜）在内的许多叶菜造成严重破坏。在这项研究中，从‘Valmaine’（抗性）和‘Okeechobee’（敏感）罗马生菜品种的种内杂交中开发了 125 个 F 家族群体，用于控制斑点损伤的抗性基因座的遗传分析和分子图谱构建。实验在昆虫饲养室（控制环境）中进行。斑点损伤被证明是可遗传的，因为广义遗传力（H）为 0.58。分离分析表明，单个显性等位基因 Sd1 位点控制对三叶草斑潜蝇的抗性。此外，数量性状位点（QTL）分析鉴定了一个新的 QTL，命名为 LG5 上的斑点（qSTP5），由两个 SNP 侧翼，映射到一个 5.2 cM（8.5 Mb 区域）的区间，解释了超过 13%的总表型方差。对三叶草斑潜蝇的抗性的有利等位基因来自抗性品种 Valmaine。鉴定与负责三叶草斑潜蝇抗性的 QTL 紧密连锁的 SNP 应有助于植物育种家开发抗性罗马生菜品种。
