Chen Zhigang, Zhou Lihua, Yuan Qiaoling, Chen Huiyu, Lei Hongyu, Su Jianming
Department of Basic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, China.
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, China.
J Biochem Mol Toxicol. 2021 Apr;35(4):e22706. doi: 10.1002/jbt.22706. Epub 2021 Jan 14.
Fumonisin B (FB ) is a common environmental mycotoxin produced by molds such as Fusarium verticillioides. The toxin poses health risks to domestic animals, including pigs, through FB -contaminanted feed. However, the cytotoxicity of FB to porcine intestines has not been fully analyzed. In the present study, the effects of FB on oxidative stress and nutrient transporter-associated genes of the porcine intestinal IPEC-J2 cells were explored. FB decreased IPEC-J2 proliferation but did not trigger reactive oxygen species (ROS) overproduction. Meanwhile, FB reduced the expression levels of the transporters l-type amino acid transporter-1 (y LAT1), solute carrier family 7 member 1 (SLC7A1), solute carrier family 1 member 5 (ASCT2), and excitatory amino acid carrier 1 (EAAC1); in addition, FB reduced the levels of the fatty acid transporters long-chain fatty acid transport protein 1 (FATP1) and long-chain fatty acid transport protein 4 (FATP4) as well as glucose transporters Na /glucose cotransporter 1 (SGLT1) and glucose transporter 2 (GLUT2). FB stimulation increased the expression levels of peptide transporter peptide transporter 1 (PepT1) and metal ion transport-related gene zinc transporter 1 (ZNT1). Moreover, metal ion transporter divalent metal transporter 1 (DMT1) expression was depressed by a higher dosage of FB . The data indicate that FB results in aberrant expression of nutrient transporters in IPEC-J2 cells, thereby exerting its toxicity even though it fails to exert ROS-dependent oxidative stress.
伏马菌素B（FB）是一种由轮枝镰孢菌等霉菌产生的常见环境霉菌毒素。这种毒素通过受FB污染的饲料对包括猪在内的家畜构成健康风险。然而，FB对猪肠道的细胞毒性尚未得到充分分析。在本研究中，探讨了FB对猪肠道IPEC-J2细胞氧化应激和营养转运蛋白相关基因的影响。FB降低了IPEC-J2细胞的增殖，但未引发活性氧（ROS）的过量产生。同时，FB降低了转运蛋白l型氨基酸转运体1（y+LAT1）、溶质载体家族7成员1（SLC7A1）、溶质载体家族1成员5（ASCT2）和兴奋性氨基酸载体1（EAAC1）的表达水平；此外，FB还降低了脂肪酸转运蛋白长链脂肪酸转运蛋白1（FATP1）和长链脂肪酸转运蛋白4（FATP4）以及葡萄糖转运蛋白钠/葡萄糖共转运体1（SGLT1）和葡萄糖转运体2（GLUT2）的水平。FB刺激增加了肽转运蛋白肽转运体1（PepT1）和金属离子转运相关基因锌转运体1（ZNT1）的表达水平。此外，较高剂量的FB抑制了金属离子转运体二价金属转运体1（DMT1）的表达。数据表明，FB导致IPEC-J2细胞中营养转运蛋白的异常表达，并因此发挥其毒性，即使它未能引发ROS依赖性氧化应激。
