Department of Environmental Machinery, Korea Institute of Machinery and Materials, Daejeon 34103, Republic of Korea.
Aerosol and Particle Technology Laboratory, Department of Mechanical Engineering, Sejong University, Seoul 05006, Republic of Korea.
Nano Lett. 2021 Jan 27;21(2):1017-1024. doi: 10.1021/acs.nanolett.0c04096. Epub 2021 Jan 14.
Bioaerosols, including infectious diseases such as COVID-19, are a continuous threat to global public safety. Despite their importance, the development of a practical, real-time means of monitoring bioaerosols has remained elusive. Here, we present a novel, simple, and highly efficient means of obtaining enriched bioaerosol samples. Aerosols are collected into a thin and stable liquid film by the unique interaction of a superhydrophilic surface and a continuous two-phase centrifugal flow. We demonstrate that this method can provide a concentration enhancement ratio of ∼2.4 × 10 with a collection efficiency of ∼99.9% and an aerosol-into-liquid transfer rate of ∼95.9% at 500 nm particle size (smaller than a single bacterium). This transfer is effective in both laboratory and external ambient environments. The system has a low limit of detection of <50 CFU/m using a straightforward bioluminescence-based technique and shows significant potential for air monitoring in occupational and public-health applications.
生物气溶胶，包括 COVID-19 等传染病，对全球公共安全构成持续威胁。尽管它们很重要，但开发一种实用的、实时的生物气溶胶监测方法仍然难以实现。在这里，我们提出了一种新颖、简单且高效的获取富集生物气溶胶样本的方法。气溶胶通过超亲水表面和连续两相离心流的独特相互作用被收集到薄而稳定的液膜中。我们证明，该方法可以在 500nm 粒径（小于单个细菌）下提供约 2.4×10 的浓度增强比、约 99.9%的收集效率和约 95.9%的气溶胶到液体的转移率。这种转移在实验室和外部环境中都很有效。该系统使用简单的基于生物发光的技术，其检测限低至<50 CFU/m，在职业和公共卫生应用中的空气监测方面具有很大的潜力。
