College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou 510642, China; Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, Guangzhou 510642, China.
Infect Genet Evol. 2021 Apr;89:104721. doi: 10.1016/j.meegid.2021.104721. Epub 2021 Jan 11.
Since 2005, subgroup J avian leukosis virus (ALV-J) infection has been present in yellow chickens in Guangdong, China, causing severe economic losses to the local poultry industry. ALV-J is a rapidly evolving retrovirus. To investigate the molecular characteristics of ALV-J isolates from yellow breeder chickens in Guangdong, 17 virus strains were isolated from 6549 anticoagulants from clinically healthy birds between 2016 and 2019, and completely sequenced and phylogenetically analyzed. Phylogenetic analysis of the gp85 gene showed that all isolated viruses were divided into three different branches. Notably, 41.2% (7/17) of the isolates shared a novel G2598A nucleotide mutation in the pol gene and caused the stop codon to be advanced by 8 positions. Nearly 200 nucleotides were deleted from the redundant TM (rTM) region in all strains, but all retained an intact direct repeat (DR1). 82.4% (14/17) of isolates contained a complete E element. Additionally, 29.4% (5/17) of isolates detected an 11 bp deletion in U3 region, and the AIB REP1 transcription factor is missing. The study indicated that ALV-J infection had still been prevalent in the yellow breeder chicken farms in Guangdong, and the genetic background of the strains is diverse. This study provides the latest data on the molecular characteristics of ALV-J, which will help to reveal the evolution trend of ALV-J and develop relevant prevention and control measures.
自 2005 年以来，中国广东的黄鸡中一直存在 J 亚群禽白血病病毒（ALV-J）感染，给当地家禽业造成了严重的经济损失。ALV-J 是一种快速进化的逆转录病毒。为了研究广东黄羽种鸡中 ALV-J 分离株的分子特征，我们从 2016 年至 2019 年期间临床健康鸡的 6549 份抗凝剂中分离出 17 株病毒，并对其进行了完全测序和系统进化分析。gp85 基因的系统进化分析显示，所有分离出的病毒分为 3 个不同的分支。值得注意的是，17 株分离株中有 41.2%（7/17）在 pol 基因中发生了新的 G2598A 核苷酸突变，导致终止密码子提前 8 位。所有毒株的冗余 TM（rTM）区都缺失了近 200 个核苷酸，但都保留了完整的直接重复（DR1）。82.4%（14/17）的分离株含有完整的 E 元件。此外，17 株分离株中有 29.4%（5/17）在 U3 区检测到 11bp 缺失，AIB REP1 转录因子缺失。研究表明，ALV-J 感染在广东黄羽种鸡场仍很普遍，毒株的遗传背景多样。本研究提供了 ALV-J 分子特征的最新数据，有助于揭示 ALV-J 的进化趋势，制定相关防控措施。
