Institute of Plant Resources, Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian Nationalities University, Dalian, 116600, China.
Institute of Plant Resources, Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian Nationalities University, Dalian, 116600, China.
Plant Physiol Biochem. 2021 Mar;160:8-17. doi: 10.1016/j.plaphy.2021.01.002. Epub 2021 Jan 6.
Yellowhorn (Xanthoceras sorbifolium Bunge) is a woody oil species that is widely distributed in northwestern China. To investigate the molecular mechanisms underlying the drought and heat tolerance response of yellowhorn seedlings, changes in protein abundance were analyzed via comparative proteomics. Drought and heat treatment of seedlings was applied in growth chamber, and the leaves were harvested after 7 days of treatment. The total protein was extracted, and comparative proteomic analysis was performed via isobaric tag for relative and absolute quantitation (iTRAQ). The abundance of most of the proteins associated with oxidative phosphorylation, NADH dehydrogenase and superoxide dismutase (SOD) was reduced. The differential proteins associated with photosynthesis enzymes indicated that stress had different effects on photosystem I (PSI) and photosystem II (PSII). After comprehensively analyzing the results, we speculated that drought and heat stress could hinder the synthesis of riboflavin, reducing NADH dehydrogenase content, which might further have an impact on energy utilization. Yellowhorn seedlings relied on Fe-Mn SOD enzymes rather than Cu/Zn SOD enzymes to remove reactive oxygen species (ROS). In addition, heat-shock proteins (HSPs) had significant increase and played a key role in stress response, which could be divided into two categories according to their transcription and translation efficiency. Over all, the results can provide a basis for understanding the molecular mechanism underlying resistance to drought and heat stress in yellowhorn and for subsequent research of posttranslational modification-related omics of key proteins.
黄杞（Xanthoceras sorbifolium Bunge）是一种广泛分布于中国西北地区的木本油料树种。为了研究黄杞幼苗对干旱和高温胁迫的响应机制，采用比较蛋白质组学方法分析了蛋白丰度的变化。在生长室中对幼苗进行干旱和高温处理，处理 7 天后收获叶片。提取总蛋白，通过同位素相对和绝对定量标记（iTRAQ）进行比较蛋白质组学分析。与氧化磷酸化、NADH 脱氢酶和超氧化物歧化酶（SOD）相关的大多数蛋白的丰度降低。与光合作用酶相关的差异蛋白表明，胁迫对光系统 I（PSI）和光系统 II（PSII）有不同的影响。综合分析结果后，我们推测干旱和高温胁迫可能会阻碍核黄素的合成，降低 NADH 脱氢酶的含量，从而进一步影响能量的利用。黄杞幼苗依赖于 Fe-Mn SOD 酶而不是 Cu/Zn SOD 酶来清除活性氧（ROS）。此外，热休克蛋白（HSPs）的表达显著增加，在应激响应中发挥关键作用，根据其转录和翻译效率可分为两类。总之，该研究结果可为理解黄杞抗旱耐热的分子机制提供依据，为后续研究关键蛋白的翻译后修饰相关组学奠定基础。
