Institute of Vegetable Research, Guangxi Academy of Agricultural Sciences, Nanning, China.
Biozeron Shenzhen, Inc, Shenzhen, China.
Mol Ecol Resour. 2021 May;21(4):1274-1286. doi: 10.1111/1755-0998.13321. Epub 2021 Feb 3.
The eggplant (Solanum melongena L.) is one of the most important Solanaceae crops, ranking third for total production and economic value in its genus. Herein, we report a high-quality, chromosome-scale eggplant reference genome sequence of 1155.8 Mb, with an N50 of 93.9 Mb, which was assembled by combining PacBio long reads and Hi-C sequencing data. Repetitive sequences occupied 70.1% of the assembly length, and 35,018 high-confidence protein-coding genes were annotated based on multiple sources. Comparative analysis revealed 646 species-specific families and 364 positive selection genes, conferring distinguishing traits on the eggplant. We performed genome-wide comparative identification of disease resistance genes and discovered an expanded gene family of bacterial spot resistance in eggplant and pepper, but not in tomato and potato. The genes involved in chlorogenic acid synthesis were comprehensively characterized. Highly similar chromosomal distribution patterns of polyphenol oxidase genes were observed in the eggplant, tomato, and potato genomes. The eggplant reference genome sequence will not only facilitate evolutionary studies of the Solanaceae but also facilitate their breeding and improvement.
茄子（Solanum melongena L.）是茄科中最重要的作物之一，在该属中总产和经济价值排名第三。本文报道了一个高质量的、染色体级别的 1155.8Mb 茄子参考基因组序列，N50 为 93.9Mb，该序列通过结合 PacBio 长读长和 Hi-C 测序数据组装得到。重复序列占据了组装长度的 70.1%，基于多个来源注释了 35018 个高可信度的蛋白质编码基因。比较分析揭示了 646 个物种特异性家族和 364 个正选择基因，赋予茄子独特的特征。我们对抗病基因进行了全基因组的比较鉴定，发现茄子和辣椒中存在一个扩展的斑点病抗性基因家族，但在番茄和马铃薯中不存在。还全面表征了绿原酸合成基因。在茄子、番茄和马铃薯基因组中观察到多酚氧化酶基因高度相似的染色体分布模式。茄子参考基因组序列不仅将促进茄科的进化研究，还将促进其育种和改良。
