Cas Lek Cesk. 2020 Winter;159(7-8):303-311.
In the nursing home in Břevnice, 21 out of 23 seniors were infected with the SARS-CoV-2 virus in the spring of 2020. Three of them died from the infection. This study monitors the antibodies formation in group of seniors who have overcome the infection. Between April and November (1-8 months after the infection), the antibodies were measured repeatedly within the period of several weeks. The virus neutralizing tests was used as well as the ECLIA assay by Roche (total immunoglobulins), CMIA assay by Abbott (IgG) and ELISA assay by Euroimmun (IgG and IgA). Six months from the infection, neutralizing antibodies were detected in 18 out of 20 seniors. High levels of neutralizing antibodies correlated with higher levels of immunoglobulins and were a good predictor of an increase of IgG in the autumn during the second wave of the epidemic in the Czech Republic. During the autumn wave, neither any of the clients, nor any staff contracted the virus, although the prevalence of PCR positivity in the Vysočina region reached around 5 %. The antigen tests also came out all negative. This study shows that in the senior population, the production of protective antibodies follows a normal pattern, and the seniors are probably immune to a repeated infection for at least 8 months from the first infection. Based on these results, we would like to open the discussion on the testing for seroprevalence in nursing homes, possible changes to the epidemiologic regime in relation to the risk of infection, and about vaccination schemes in these centers.
在 2020 年春季，布尔诺的贝夫尼采养老院 23 名老年人中有 21 人感染了 SARS-CoV-2 病毒。其中 3 人因感染而死亡。本研究监测了已感染老年人的抗体形成情况。在 4 月至 11 月（感染后 1-8 个月）期间，在数周内多次重复测量了抗体。该研究使用了病毒中和试验以及罗氏公司的 ECLIA 检测（总免疫球蛋白）、雅培公司的 CMIA 检测（IgG）和 Euroimmun 公司的 ELISA 检测（IgG 和 IgA）。在感染后 6 个月，20 名老年人中有 18 名检测到中和抗体。高水平的中和抗体与高水平的免疫球蛋白相关，并且是秋季在捷克共和国第二次疫情期间 IgG 增加的良好预测指标。在秋季疫情期间，尽管 Vysočina 地区的 PCR 阳性率达到了约 5%，但养老院没有客户或工作人员感染该病毒，抗原检测也均为阴性。本研究表明，在老年人中，保护性抗体的产生遵循正常模式，老年人在第一次感染后至少 8 个月内可能对再次感染具有免疫力。基于这些结果，我们希望就养老院的血清流行率检测、与感染风险相关的流行病学政策可能的变化以及这些中心的疫苗接种计划展开讨论。
