Szkudelska Katarzyna, Okulicz Monika, Szkudelski Tomasz
Department of Animal Physiology, Biochemistry and Biostructure, Poznań University of Life Sciences, Wołyńska 35, 60-637, Poznań, Poland.
Environ Sci Pollut Res Int. 2021 May;28(18):23301-23309. doi: 10.1007/s11356-021-12411-0. Epub 2021 Jan 14.
Bisphenol A (BPA) is an ubiquitous synthetic chemical exerting numerous adverse effects. Results of rodent studies show that BPA negatively affects adipose tissue. However, the short-term influence of this compound addressing adipocyte metabolism and adipokine secretion is unknown. In the present study, isolated rat adipocytes were exposed for 2 h to 1 and 10 nM BPA. Insulin-induced glucose conversion to lipids along with glucose transport was significantly increased in the presence of BPA. However, basal glucose conversion to lipids, glucose oxidation, and formation of lipids from acetate were unchanged in adipocytes incubated with BPA. It was also shown that BPA significantly increases lipolytic response of adipocytes to epinephrine. However, lipolysis stimulated by dibutyryl-cAMP (a direct activator of protein kinase A) and the antilipolytic action of insulin were not affected by BPA. Moreover, BPA did not influence leptin and adiponectin secretion from adipocytes. Our new results show that BPA is capable of disturbing processes related to lipid accumulation in isolated rat adipocytes. This is associated with the potentiation of insulin and epinephrine action. The effects of BPA appear already after short-term exposure to low doses of this compound. However, BPA fails to change adipokine secretion.
双酚A（BPA）是一种普遍存在的合成化学物质，会产生多种不良影响。啮齿动物研究结果表明，BPA对脂肪组织有负面影响。然而，这种化合物对脂肪细胞代谢和脂肪因子分泌的短期影响尚不清楚。在本研究中，将分离的大鼠脂肪细胞暴露于1 nM和10 nM的BPA中2小时。在BPA存在的情况下，胰岛素诱导的葡萄糖转化为脂质以及葡萄糖转运显著增加。然而，在与BPA孵育的脂肪细胞中，基础葡萄糖转化为脂质、葡萄糖氧化以及由乙酸盐形成脂质的过程并未改变。研究还表明，BPA显著增加脂肪细胞对肾上腺素的脂解反应。然而，二丁酰环磷腺苷（蛋白激酶A的直接激活剂）刺激的脂解作用以及胰岛素的抗脂解作用不受BPA影响。此外，BPA不影响脂肪细胞中瘦素和脂联素的分泌。我们的新结果表明，BPA能够干扰分离的大鼠脂肪细胞中与脂质积累相关的过程。这与胰岛素和肾上腺素作用的增强有关。BPA在短期暴露于低剂量该化合物后就会出现这些效应。然而，BPA不会改变脂肪因子的分泌。
