子痫前期患者胎盘、血清及脐静脉血中帕金森病蛋白 7 的表达及意义。

Expression and significance of Parkinson disease protein 7 in placental, serum and umbilical cord blood in preeclampsia.

机构信息

Department of Obstetrics and Gynecology, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fujian, China.

出版信息

Ginekol Pol. 2020;91(12):764-768. doi: 10.5603/GP.a2020.0131.

DOI:10.5603/GP.a2020.0131

PMID:33447996

Abstract

OBJECTIVES

To study the role of changes in the expression of human Parkinson's disease protein 7 (PARK7/DJ-1) in preeclampsia.

MATERIAL AND METHODS

We selected 120 gravidas, including 60 cases of severe preeclampsia group and control group, and divided into early onset preeclampsia group (< 34 weeks), late onset preeclampsia group (≥ 34 weeks) and control group according to the onset of pregnancy. The expression level of DJ-1 was detected by ELISA. The expression level of DJ-1 in placenta tissue of gravidas was detected by Western-blot and RT-PCR.

RESULTS

The level of DJ-1 in serum and cord blood of preeclampsia group was higher than that of control group. The relative level of DJ-1 protein and DJ-1 mRNA in placenta tissue of preeclampsia group was higher than that of control group.

CONCLUSIONS

The expression level of DJ-1 in serum, umbilical cord blood and placenta tissue increased in preeclampsia patients, suggesting that DJ-1 may take part in the pathophysiology process of preeclampsia.

摘要

目的

研究人类帕金森病蛋白 7（PARK7/DJ-1）表达变化在子痫前期中的作用。

材料和方法

我们选择了 120 名孕妇，包括 60 例重度子痫前期组和对照组，并根据妊娠发病时间分为早发型子痫前期组（<34 周）、晚发型子痫前期组（≥34 周）和对照组。采用 ELISA 法检测 DJ-1 的表达水平。采用 Western-blot 和 RT-PCR 法检测孕妇胎盘组织中 DJ-1 的表达水平。