Chen Qingqing, Shan Chaowen, Su Jie, Chen Wei, Zhu Jiaming, Chen Suhong, Lyu Guiyuan
College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 311400, China.
Hangzhou Huqingyutang Pharmaceutical Co. Ltd, Hangzhou 310016, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2020 Dec 25;49(6):697-704. doi: 10.3785/j.issn.1008-9292.2020.12.04.
To investigate the effect of Chinese medicine Wubi Shanyao pills on sexual function of kidney-yang-deficiency mice induced by hydrocortisone.
Male Kunming mice were injected with hydrocortisone for 10 days to prepare the kidney-yang-deficiency model, and administrated with Wubi Shanyao pills (0.91, 1.82, 2.73 g/kg) for 9 weeks. The general behaviors of mice (autonomous activity, grasping power) were observed; sexual behaviors (capture, straddle, ejaculation frequency and incubation period) of mice were detected by mating experiment. The serum levels of cortisol, adrenocorticotropic hormone (ACTH), luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E) and testosterone were measured by ELIZA. The organ indexes of kidney, epididymis, testis and seminal vesicle were calculated. The histomorphology of testis was observed by HE staining. The apoptosis of testis cells was detected by TUNEL; and the expression level of proliferating cell nuclear antigen (PCNA) in testis was detected by immunohistochemistry.
Wubi Shanyao pills increased the number of independent activities, grasping power, capture frequency of model mice and shortened the capture latency (all <0.01); and also increased the serum levels of testosterone, cortisol, ACTH, decreased the serum levels of FSH (<0.05 or <0.01); increased testis index (<0.05), improved the pathological changes of testis, inhibited cell apoptosis, and promoted PCNA protein expression in testis.
Wubi Shanyao pills can improve the sexual function of mice with kidney-yang-deficiency induced by hydrocortisone, which may be related to regulating the hypothalamus-pituitary-adrenal axis (HPA axis), promoting the proliferation of testicular cells, and inhibiting cell apoptosis.
探讨中药无比山药丸对氢化可的松致肾阳虚小鼠性功能的影响。
雄性昆明小鼠注射氢化可的松10天制备肾阳虚模型，给予无比山药丸（0.91、1.82、2.73 g/kg）灌胃9周。观察小鼠一般行为（自主活动、抓力）；通过交配实验检测小鼠性行为（捕捉、跨骑、射精频率和潜伏期）。采用酶联免疫吸附测定法（ELISA）检测血清皮质醇、促肾上腺皮质激素（ACTH）、黄体生成素（LH）、卵泡刺激素（FSH）、雌二醇（E）和睾酮水平。计算肾脏、附睾、睾丸和精囊的脏器指数。通过苏木精-伊红（HE）染色观察睾丸组织形态学。采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法（TUNEL）检测睾丸细胞凋亡；采用免疫组织化学法检测睾丸中增殖细胞核抗原（PCNA）的表达水平。
无比山药丸可增加模型小鼠自主活动次数、抓力、捕捉频率，缩短捕捉潜伏期（均P<0.01）；还可提高血清睾酮、皮质醇、ACTH水平，降低血清FSH水平（P<0.05或P<0.01）；增加睾丸指数（P<0.05），改善睾丸病理变化，抑制细胞凋亡，促进睾丸中PCNA蛋白表达。
无比山药丸可改善氢化可的松致肾阳虚小鼠的性功能，其机制可能与调节下丘脑-垂体-肾上腺轴（HPA轴）、促进睾丸细胞增殖及抑制细胞凋亡有关。
