Department of Chemistry, University of Turku, Vatselankatu 2, 20014, Turku, Finland.
Chembiochem. 2021 May 14;22(10):1761-1764. doi: 10.1002/cbic.202000799. Epub 2021 Feb 24.
A water-soluble arylmercury complex has been synthesized, and its ability to catalyze the cleavage of the phosphodiester linkage of the RNA model compound adenylyl-3',5'-(2',3'-O-methyleneadenosine) has been assessed over a pH range of 3-8.5 and a catalyst concentration range of 0-7 mM. In the presence of 1 mM catalyst, the observed pH-rate profile featured a new pH-independent region between pH 6 and 7, the catalyzed reaction being as much as eight times faster than the background reaction. At pH 7, the acceleration increased linearly from three- to 17-fold upon increasing the catalyst concentration from 1 to 7 mM. The linear dependence indicates a relatively low affinity of the catalyst for the substrate and, hence, the potential for considerable improvement on tethering to an appropriate targeting group, such as an oligonucleotide.
已合成了一种水溶性芳基汞配合物，并在 pH 值为 3-8.5 和催化剂浓度为 0-7mM 的范围内评估了其催化 RNA 模型化合物腺苷酰基-3'，5'-(2'，3'-O-亚甲基腺苷)的磷酸二酯键断裂的能力。在 1mM 催化剂存在下，观察到的 pH-速率曲线在 pH 6 和 7 之间呈现出新的 pH 独立区域，催化反应比背景反应快多达 8 倍。在 pH 7 时，随着催化剂浓度从 1mM 增加到 7mM，加速作用从 3 倍线性增加到 17 倍。线性依赖性表明催化剂与底物的亲和力相对较低，因此，通过将其连接到适当的靶向基团（例如寡核苷酸），仍有很大的改进空间。
