Department of Chemical Engineering, Faculty of Engineering, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
Applied biotechnology research center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
J Biomed Mater Res A. 2021 Jun;109(6):981-993. doi: 10.1002/jbm.a.37087. Epub 2020 Sep 8.
Scaffolds are the important part of the tissue-engineering field that are made from different biomaterials using various techniques. In this study, new scaffold based on polycaprolactone (PCL) and poly (hydroxybutyrate-co-hydroxyvalerate) (PHBV) containing hydroxyapatite nanopraticles (n-HA) were fabricated using the dual-leaching technique (DLT). Morphology, porosity, degradation rate, Fourier transfer infrared ray (FTIR) spectra, surface, and mechanical properties as well as capacity of cell binding and cell proliferation on the constructed scaffolds were evaluated. FTIR analysis showed that n-HA particles have some interest interactions with polymeric chains. The best 3D-structure was seen in PCL70PHBV30 scaffold using the scanning electron microscopy (SEM) and its structure improved in the presence of 3, 5 wt% of n-HA. Results of energy dispersive x-ray analysis (EDXA, map of Ca) showed that the nanoparticles have the uniform distribution within the fabricated scaffolds. Porosity analysis showed that the particulate salt leaching technique is a successful approach to building a 3D structure. Increasing of PHBV content and n-HA up to 3 and 5 wt% in the PCL matrix led to increase porosity in all samples. Mechanical properties analysis showed that values of compression modulus and strength are decreased with addition of PHBV and HA nanoparticles. These results were directly in line with the results of morphology and porosity. Cell culture experiments demonstrated that the PCL/PHBV/nHA nanocomposite scaffold has a better tendency of proliferation to cells than that of the pure PCL/PHBV scaffold. All of these results suggest promising potentials of the developed PCL/PHBV/nHA scaffolds in this study desire for bone tissue engineering.
支架是组织工程领域的重要组成部分，它由不同的生物材料通过各种技术制成。在本研究中，采用双重浸出技术（DLT）制备了一种新型的基于聚己内酯（PCL）和聚（羟基丁酸-co-羟基戊酸）（PHBV）的支架，其中含有纳米羟基磷灰石（n-HA）颗粒。对构建的支架的形貌、孔隙率、降解率、傅里叶变换红外光谱（FTIR）谱、表面和力学性能以及细胞结合和增殖能力进行了评估。FTIR 分析表明，n-HA 颗粒与聚合物链之间存在一些有趣的相互作用。扫描电子显微镜（SEM）显示，PCL70PHBV30 支架具有最佳的 3D 结构，并且在存在 3、5wt%n-HA 的情况下其结构得到改善。能量色散 X 射线分析（EDXA，Ca 映射）的结果表明，纳米颗粒在制备的支架内均匀分布。孔隙率分析表明，颗粒盐浸出技术是构建 3D 结构的成功方法。在 PCL 基体中增加 PHBV 和 n-HA 的含量至 3 和 5wt%，会导致所有样品的孔隙率增加。力学性能分析表明，随着 PHBV 和 HA 纳米颗粒的加入，压缩模量和强度值降低。这些结果与形态和孔隙率的结果直接一致。细胞培养实验表明，与纯 PCL/PHBV 支架相比，PCL/PHBV/nHA 纳米复合材料支架对细胞的增殖具有更好的趋势。所有这些结果表明，所开发的 PCL/PHBV/nHA 支架在骨组织工程方面具有很大的潜力。
