Wu Mi, Li Zhao, Yao Jinrong, Shao Zhengzhong, Chen Xin
State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Laboratory of Advanced Materials, Fudan University, Shanghai 200433, People's Republic of China.
ACS Biomater Sci Eng. 2019 Sep 9;5(9):4799-4807. doi: 10.1021/acsbiomaterials.9b00794. Epub 2019 Aug 7.
Developments in technology such as theranostic nanoplatforms, which combine complementary imaging modalities and synergistic therapies, have brought new hope to the diagnosis and treatment of cancer, one of the most deadly diseases. In this article, a nanoplatform (AuNCs/PPI-ICG nanohybrid) with a theranostic effect was prepared using indocyanine green (ICG), a cyanine dye with bright near-infrared fluorescence (NIRF) and excellent photodynamic and photothermal performance, and it was loaded onto gold nanoclusters/pea protein isolate hybrid nanoparticles (AuNCs/PPI NPs). There was a linear relationship between ICG loading ratio and the concentration, within a concentration range of 0-5 mg/mL, making it possible to precisely control the concentration of loaded ICG. In addition, the AuNCs/PPI-ICG nanohybrid displayed outstanding loading stability and enhanced the aqueous stability of ICG, owing to the protectivity of AuNCs/PPI NPs. cell experiments showed that this biocompatible nanohybrid could be taken up by A549 cells and used as a fluorescent bioimaging probe. Moreover, because of Au, AuNCs/PPI NPs could also act as a potential computed tomographic (CT) contrast agent, and so the AuNCs/PPI-ICG nanohybrid had the potential to function as a dual-modal imaging agent. Finally, the as-prepared hybrid, with excellent photodynamic properties and exceptional photothermal capabilities, showed a satisfactory therapeutic effect in ablating A549 cancer cells. These results convincingly demonstrate that the AuNCs/PPI-ICG nanohybrid is a promising theranostic nanoplatform for NIRF/CT dual-modal imaging and for synergistic photodynamic therapy/photothermal therapy (PDT/PTT).
诸如治疗诊断纳米平台等技术的发展，将互补成像模式与协同疗法相结合，为癌症（最致命的疾病之一）的诊断和治疗带来了新希望。在本文中，使用吲哚菁绿（ICG）制备了具有治疗诊断效果的纳米平台（金纳米簇/豌豆蛋白分离物-吲哚菁绿纳米杂化物），吲哚菁绿是一种具有明亮近红外荧光（NIRF）以及优异光动力和光热性能的花菁染料，并将其负载到金纳米簇/豌豆蛋白分离物杂化纳米颗粒（金纳米簇/豌豆蛋白纳米颗粒）上。在0 - 5 mg/mL的浓度范围内，ICG负载率与浓度之间存在线性关系，这使得精确控制负载ICG的浓度成为可能。此外，由于金纳米簇/豌豆蛋白纳米颗粒的保护作用，金纳米簇/豌豆蛋白分离物-吲哚菁绿纳米杂化物表现出出色的负载稳定性，并增强了ICG的水稳定性。细胞实验表明，这种生物相容性纳米杂化物可被A549细胞摄取并用作荧光生物成像探针。此外，由于金的存在，金纳米簇/豌豆蛋白纳米颗粒还可作为潜在的计算机断层扫描（CT）造影剂，因此金纳米簇/豌豆蛋白分离物-吲哚菁绿纳米杂化物有潜力作为双模态成像剂发挥作用。最后，所制备的杂化物具有优异的光动力性能和出色的光热能力，在消融A549癌细胞方面显示出令人满意的治疗效果。这些结果令人信服地证明，金纳米簇/豌豆蛋白分离物-吲哚菁绿纳米杂化物是一种有前途的治疗诊断纳米平台，可用于近红外荧光/计算机断层扫描双模态成像以及协同光动力疗法/光热疗法（PDT/PTT）。
