Department of Genetics, Federal University of Pernambuco UFPE, Recife, Pernambuco, Brazil.
Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco UFPE, Recife, Pernambuco, 50670-901, Brazil.
Immunogenetics. 2021 Aug;73(4):349-353. doi: 10.1007/s00251-020-01202-5. Epub 2021 Jan 15.
Pyroptosis cell death in recent thymus emigrants (RTE) CD4+ T lymphocytes plays an important role on HIV-1 infection as a cause of CD4+ T cell depletion, being influenced by several factors, among them, the sex. Thus, the aim of this study was evaluated pyroptosis levels in RTE CD4+ T lymphocytes of individuals under antiretroviral therapy (ART) stratified by sex. Thirty-seven ART-treated HIV-positive patients (22 females and 15 males) and 12 (seven females and five males) clinically health subjects were recruited. Analysis by flow-cytometry of RTE CD4+ cells (CD4+ CD31+ /fluorescent-labeled inhibitors of caspases-Caspase-1+) were performed. Clinical and sociodemographic aspects were also evaluated from medical records. We observed statistically higher levels of pyroptosis RTE CD4+ T cells in male individuals (69.3%) compared with female group (39.1%) (P = 0.0356). Pre- and post-treatment CD4+ T cell counts were also higher in women than men (P = 0.004 and P = 0.012, respectively). Our data provides important evidence of the sex as a potential predictor of immunological reconstitution in ART-treated individuals.
新近胸腺迁出（RTE）CD4+T 淋巴细胞的细胞焦亡在 HIV-1 感染中作为 CD4+T 细胞耗竭的原因起重要作用，受多种因素影响，其中包括性别。因此，本研究旨在评估接受抗逆转录病毒治疗（ART）的个体中 RTE CD4+T 淋巴细胞的细胞焦亡水平，并按性别分层。招募了 37 名接受 ART 治疗的 HIV 阳性患者（22 名女性和 15 名男性）和 12 名（7 名女性和 5 名男性）临床健康受试者。通过流式细胞术分析 RTE CD4+细胞（CD4+CD31+荧光标记的半胱天冬酶抑制剂-胱天冬酶-1+）。还从病历中评估了临床和社会人口统计学方面。我们观察到男性个体（69.3%）的 RTE CD4+T 细胞细胞焦亡水平明显高于女性组（39.1%）（P=0.0356）。女性的治疗前和治疗后 CD4+T 细胞计数也高于男性（P=0.004 和 P=0.012）。我们的数据提供了重要证据，表明性别是接受 ART 治疗的个体免疫重建的潜在预测因素。
