长链非编码 RNA SNHG8 通过抑制 miR-384 和上调 HOXB7 促进前列腺癌的进展。
Long non-coding RNA SNHG8 promotes prostate cancer progression through repressing miR-384 and up-regulating HOXB7.
机构信息
Department of Urology Center, People's Hospital of Xinjiang Uygur Autonomous Region, Xinjiang, China.
Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.
出版信息
J Gene Med. 2021 Mar;23(3):e3309. doi: 10.1002/jgm.3309. Epub 2021 Feb 23.
BACKGROUND
Multiple long non-coding RNAs (lncRNAs) have been demonstrated to function as vital regulators in the progression of prostate cancer (PCa). In the present study, we aimed to probe the function of lncRNA small nucleolar RNA host gene 8 (SNHG8) in PCa progression.
METHODS
A quantitative real-time polymerase chain reaction and western blotting were utilized to measure SNHG8, microRNA-384 (miR-384) and homeobox B7 (HOXB7) expression. Call-couting kit-8 and bromodeoxyuridine experiments were employed to evaluate PCa cell proliferation. Transwell experiments were performed to detect PCa cell migration and invasion. Dual-luciferase reporter experiments and RNA immunoprecipitation experiments were conducted to determine the targeting relationships among miR-384, SNHG8 and HOXB7.
RESULTS
SNHG8 was up-regulated in PCa tissues and cells. Silencing of SNHG8 suppressed the proliferation, migration and invasion of PCa cells. SNHG8 functioned as a molecular sponge to repress miR-384. The effects of SNHG8 knockdown on PCa cell proliferation, migration and invasion were counteracted by miR-384 inhibition. HOXB7 was confirmed to be a target gene of miR-384. SNHG8 knockdown repressed HOXB7 expression via targeting miR-384.
CONCLUSIONS
SNHG8 promotes PCa cell proliferation, migration and invasion via decoying miR-384 and up-regulating HOXB7.
背景
多项长链非编码 RNA（lncRNA）已被证明在前列腺癌（PCa）的进展中作为重要的调节因子发挥作用。在本研究中，我们旨在探讨 lncRNA 小核仁 RNA 宿主基因 8（SNHG8）在 PCa 进展中的功能。
方法
利用实时定量聚合酶链反应和蛋白质印迹法测定 SNHG8、微小 RNA-384（miR-384）和同源盒 B7（HOXB7）的表达。细胞计数试剂盒-8 和溴脱氧尿苷实验用于评估 PCa 细胞增殖。Transwell 实验用于检测 PCa 细胞迁移和侵袭。双荧光素酶报告基因实验和 RNA 免疫沉淀实验用于确定 miR-384、SNHG8 和 HOXB7 之间的靶向关系。
结果
SNHG8 在 PCa 组织和细胞中上调。沉默 SNHG8 抑制了 PCa 细胞的增殖、迁移和侵袭。SNHG8 作为分子海绵抑制 miR-384。miR-384 抑制可逆转 SNHG8 敲低对 PCa 细胞增殖、迁移和侵袭的影响。HOXB7 被证实是 miR-384 的靶基因。SNHG8 通过靶向 miR-384 抑制 HOXB7 的表达。
结论
SNHG8 通过充当 miR-384 的诱饵并上调 HOXB7 促进 PCa 细胞的增殖、迁移和侵袭。
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