Institute of Translational Medicine, Medical College, Yangzhou University, The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou 225001, China.
Department of Pharmacy, College of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, China.
Life Sci. 2021 Mar 15;269:119021. doi: 10.1016/j.lfs.2021.119021. Epub 2021 Jan 12.
The recurrence and metastasis of gastric cancer has always been an important factor affecting the prognosis of gastric cancer. Cancer stem cells can promote the recurrence and growth of gastric cancer. The identification and isolation of gastric cancer stem cells contribute to the origin, progress and treatment strategy of gastric cancer. The aim of this study was to identify and isolate gastric cancer stem cells, and provide targets for the treatment of gastric cancer.
Magnetic-activated cell sorting was used to isolate CD133+/CD166+ cell populations from human gastric adenocarcinoma cell lines (BGC-823 and SGC-7901). Sphere formation, cell proliferation, resistance to chemotherapy, colony formation, migration invasion and tumorigenicity in vivo of these cell populations were evaluated. Moreover, RT-qPCR and Western blot were used to investigate the expression level of the stem cell markers Nanog, Sox2, Oct-4, and c-Myc.
CD133+/CD166+ cell subpopulations presented more malignant features than CD133-/CD166-, CD133-/CD166+, CD133+/CD166- cell populations and parental cells. Moreover, the mRNA and protein expression level of Oct-4 and c-Myc were higher in CD133+/CD166+ cells than in parental cells or other cell populations.
The CD133+/CD166+ populations of human gastric cancer cell lines BGC-823 and SGC-7901 have cancer stem cell characteristics.
胃癌的复发和转移一直是影响胃癌预后的重要因素。肿瘤干细胞可以促进胃癌的复发和生长。胃癌干细胞的鉴定和分离有助于胃癌的起源、进展和治疗策略。本研究旨在鉴定和分离胃癌干细胞，为胃癌的治疗提供靶点。
采用磁激活细胞分选技术从人胃腺癌细胞系（BGC-823 和 SGC-7901）中分离出 CD133+/CD166+细胞群。评估这些细胞群的球体形成、细胞增殖、化疗耐药性、集落形成、迁移侵袭和体内致瘤性。此外，采用 RT-qPCR 和 Western blot 检测干细胞标志物 Nanog、Sox2、Oct-4 和 c-Myc 的表达水平。
CD133+/CD166+细胞亚群比 CD133-/CD166-、CD133-/CD166+、CD133+/CD166-细胞群和亲本细胞具有更多的恶性特征。此外，CD133+/CD166+细胞中 Oct-4 和 c-Myc 的 mRNA 和蛋白表达水平高于亲本细胞或其他细胞群。
人胃腺癌细胞系 BGC-823 和 SGC-7901 中的 CD133+/CD166+细胞群具有肿瘤干细胞特征。
