Bengbu Medical College, Bengbu 233030, PR China.
Fuzong Clinical Medical College of Fujian Medical University, Fuzhou 350025, PR China.
J Neurosci Methods. 2021 Mar 1;351:109075. doi: 10.1016/j.jneumeth.2021.109075. Epub 2021 Jan 12.
To construct a new and improved model of acute subdural hematoma in rats.
30 male adult Sprague-Dawley rats(SD rats) were selected and randomly divided into two groups. The traditional model group was based on Miller's model construction method, and the improved model group was based on improved needle, injection site and operation method. The improved model was evaluated by comparing the physiological indicators, behavioral scores, magnetic resonance performance and HE staining results of the two groups of rats.
The physical signs of the rats in the two groups were similar. The survival rate of the improved group was higher than that of the traditional group. The hematoma in the improved model was thicker and concentrated in the ipsilateral side, as revealed by HE staining and MRI. The improved method has less intrusions on the cortex around the injection site and is more stable than the traditional model.
COMPARISON WITH EXISTING METHOD(S): The operation difficulty of the improved model is reduced and easier. The survival rate of the improved group was higher than that of the traditional group. And the improved model will have more research possibilities.
The improved model is based on the traditional model. Although it has some shortcomings, it can also be used in different research fields of the traditional model. The operation for the improved model is easier to perform. And the improved model has more applications in research.
构建一种新的、改良的大鼠急性硬膜下血肿模型。
选择 30 只成年雄性 Sprague-Dawley 大鼠（SD 大鼠），随机分为两组。传统模型组基于 Miller 模型构建方法，改良模型组基于改良后的针、注射部位和操作方法。通过比较两组大鼠的生理指标、行为评分、磁共振表现和 HE 染色结果来评估改良模型。
两组大鼠的体征相似。改良组的存活率高于传统组。HE 染色和 MRI 显示，改良模型中的血肿更厚，更集中在同侧。改良方法对注射部位周围皮质的侵入性较小，比传统模型更稳定。
改良模型的操作难度降低，且更简单。改良组的存活率高于传统组。改良模型将具有更多的研究可能性。
改良模型是在传统模型的基础上建立的。虽然它有一些缺点，但也可以应用于传统模型的不同研究领域。改良模型的操作更容易进行。而且改良模型在研究中有更多的应用。
