Fisheries Science Institute, Chonnam National University, Yeosu, 59626, South Korea; Department of Biotechnology & Microbiology, National College, Tiruchirappalli, 620001, India; Department of Animal Health and Management, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India.
Fisheries Science Institute, Chonnam National University, Yeosu, 59626, South Korea; Department of Biotechnology & Microbiology, National College, Tiruchirappalli, 620001, India.
Carbohydr Res. 2021 Jan;499:108224. doi: 10.1016/j.carres.2020.108224. Epub 2020 Dec 30.
A mannose binding lectin (C-type lectin) was detected in a molluscan snail Hemifusus pugilinus, this lectin molecule was isolated and purified from the plasma using mannose-fixed sepharose CL-4B column affinity chromatography. The purified protein corresponds to the molecular weight of 118 kDa on an SDS-PAGE gel. The divalent cation-dependent nature of the H. pugilinus lectin (Hp-Lec) evidenced through pH and thermal stability analysis using Circular Dichroism (CD) and Surface Plasmon Resonance (SPR) respectively. Functional investigations of the Hp-Lec reveal a broad spectrum of bacterial agglutination activity against wide range of Gram-positive and Gram-negative bacterial strains. Furthermore, Hp-Lec displayed the haemo agglutination activity against vertebrate red blood cells (RBCs) and its titers were recorded. Excitingly, microbial virulent pathogens such as fungal strains tested against the purified Hp-Lec (25 and 50 μg/ml), which exhibits the effective antifungal activity against tested fungal pathogens such as Aspergillus niger and A. flavus.
在一种软体动物芋螺 Hemifusus pugilinus 中检测到甘露糖结合凝集素（C 型凝集素），该凝集素分子是通过使用甘露糖固定琼脂糖 CL-4B 柱亲和层析从血浆中分离和纯化得到的。纯化的蛋白质在 SDS-PAGE 凝胶上对应于 118 kDa 的分子量。通过圆二色性（CD）和表面等离子体共振（SPR）分别进行 pH 和热稳定性分析，证明了芋螺凝集素（Hp-Lec）的二价阳离子依赖性。对 Hp-Lec 的功能研究表明，它对广泛的革兰氏阳性和革兰氏阴性细菌菌株具有广谱的细菌凝集活性。此外，Hp-Lec 对脊椎动物红细胞（RBC）具有血凝集活性，并且记录了其效价。令人兴奋的是，对纯化的 Hp-Lec（25 和 50 μg/ml）测试了微生物毒力病原体，如真菌株，显示出对测试真菌病原体如黑曲霉和黄曲霉的有效抗真菌活性。
