Pham Lan Doan, Do Duy Ngoc, Nam Le Quang, Van Ba Nguyen, Ninh Pham Hai, Thuy Doan Phuong, Son Pham Van, Thieu Pham Cong
Key Laboratory of Animal Cell Technology, National Institute of Animal Sciences, Hanoi, Vietnam.
Institute of Research and Development, Duy Tan University, Danang, Vietnam.
Anim Biotechnol. 2022 Nov;33(6):1065-1072. doi: 10.1080/10495398.2020.1868485. Epub 2021 Jan 15.
This study characterized genetic diversity and population structure of four indigenous Vietnamese duck breeds and an exotic breed for setting the conservation priority. A total of 200 samples from four duck breeds ( and ) and an exotic breed () were genotyped for fifteen microsatellite markers. The average number of alleles per locus was 14.07. A moderate genetic diversity was observed for indigenous breeds as mean of observed and expected heterozygosity as Ho = 0.50 and He = 0.57, respectively. The had the lowest values of Ho (0.41) and He (0.48) while had the highest values of Ho (0.6) and He (0.69), respectively. The inbreeding coefficients () ranged from 0.12 to 0.16, and all breeds were significantly under heterozygote deficit. Nei's genetic distance was the shortest between and . The discriminant analysis of principal components of studied breeds resulted in four genetic clusters. The and breeds joined the same genetic cluster while other breeds had their own clusters. These results indicated that the possibility to combine and for reducing the cost of conservation and suggested that conservation of the should be prioritized to avoid inbreeding depression and genetic drift.
本研究对四个越南本土鸭品种和一个外来品种的遗传多样性和种群结构进行了表征，以确定保护优先级。对来自四个鸭品种（和）和一个外来品种（）的总共200个样本进行了15个微卫星标记的基因分型。每个位点的平均等位基因数为14.07。本土品种观察到中等程度的遗传多样性，观察杂合度和期望杂合度的平均值分别为Ho = 0.50和He = 0.57。的Ho值（0.41）和He值（0.48）最低，而的Ho值（0.6）和He值（0.69）最高。近交系数（）范围为0.12至0.16，所有品种均显著存在杂合子缺失。和之间的Nei遗传距离最短。对所研究品种的主成分判别分析产生了四个遗传簇。和品种归入同一遗传簇，而其他品种有各自的簇。这些结果表明，将和结合起来以降低保护成本是有可能的，并建议应优先保护以避免近交衰退和遗传漂变。
