Tianjin Key Laboratory of Micro-scale Optical Information Science and Technology, and Institute of Modern Optics, Nankai University, Tianjin, China.
State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, China.
J Biophotonics. 2021 May;14(5):e202000466. doi: 10.1002/jbio.202000466. Epub 2021 Jan 25.
Light sheet fluorescence microscopy has become a research hotspot in biomedicine because of low phototoxicity, high speed, and high resolution. However, the conventional methods to acquire three-dimensional spatial information are mainly based on scanning, which inevitably increases photodamage and is not real-time. Here, we propose a method to generate controllable multi-planar illumination with a dielectric isosceles triangular array and a design of multi-planar light sheet fluorescence microscopy system. We carry out experiments of three-dimensional illumination beam measurement, volumetric imaging of fluorescent microspheres, and dynamic in vivo imaging of zebrafish heart to evaluate the performance of this system. In addition, we apply this system to study the effects of bisphenol fluorene on the heart shape and heart-beating rate of zebrafish. Our experiment results indicate that the multi-planar light sheet microscopy system provides a novel and feasible method for three-dimensional selected plane imaging and low-phototoxicity in vivo imaging.
光片荧光显微镜由于其低光毒性、高速和高分辨率，已成为生物医学领域的研究热点。然而，传统的获取三维空间信息的方法主要基于扫描，这不可避免地增加了光损伤，并且不是实时的。在这里，我们提出了一种利用介电等腰三角形阵列产生可控多平面照明的方法，并设计了多平面光片荧光显微镜系统。我们进行了三维照明光束测量、荧光微球的体积成像以及斑马鱼心脏的动态活体成像实验，以评估该系统的性能。此外，我们将该系统应用于研究双酚芴对斑马鱼心脏形状和心跳率的影响。我们的实验结果表明，多平面光片显微镜系统为三维选择平面成像和低光毒性活体成像提供了一种新颖且可行的方法。
