State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400716, China.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
Int J Biol Macromol. 2021 Mar 1;172:223-230. doi: 10.1016/j.ijbiomac.2021.01.021. Epub 2021 Jan 13.
Microsporidia are obligate single-celled eukaryote parasites. Microsporidian infection can cause large economic losses to beneficial insects such as silkworms and honey bees. Identification of resistance biomacromolecules and breeding of transgenic lines resistant to the microsporidian Nosema bombycis are important for disease management. We previously used transcriptome analysis to identify a guanylate binding protein family BmAtlastin-n gene that was significantly upregulated after Nosema bombycis infection, and we determined that the molecule was highly expressed in resistance-related tissues such as the midgut, fat body and the epidermis. The transgenic silkworm line overexpressing BmAtlastin-n biomolecules had economic characters similar to those of non-transgenic lines. The transgenic OE-BmAtlastin-n lines had significantly improved survival after microspore infection. We used RT-PCR and H&E staining to show that the number of spores in the transgenic lines was significantly lower than in the control lines. In this study, we identified a BmAtlastin-n macromolecule with resistance to N. bombycis and developed a transgenic line. The results improved understanding of the GBP protein family and provided biomacromolecule material for the treatment and prevention of microsporidia.
微孢子虫是专性单细胞真核寄生虫。微孢子虫感染会给家蚕和蜜蜂等有益昆虫造成巨大的经济损失。鉴定抗微孢子虫Nosema bombycis 的抗性生物大分子并培育转基因品系对于疾病管理非常重要。我们之前使用转录组分析鉴定了一个鸟苷酸结合蛋白家族 BmAtlastin-n 基因，该基因在 Nosema bombycis 感染后显著上调，我们确定该分子在与抗性相关的组织（如中肠、脂肪体和表皮）中高度表达。过表达 BmAtlastin-n 生物大分子的转基因家蚕系具有与非转基因系相似的经济特征。过表达 OE-BmAtlastin-n 的转基因系在微孢子感染后存活率显著提高。我们使用 RT-PCR 和 H&E 染色表明，转基因系中的孢子数量明显低于对照系。在这项研究中，我们鉴定了一种对 N. bombycis 具有抗性的 BmAtlastin-n 大分子，并开发了一种转基因系。研究结果提高了对 GBP 蛋白家族的认识，并为微孢子虫的治疗和预防提供了生物大分子材料。
