Fang Xu'an, Tong Yuan-Jun, Li Nan, Yu Lu-Dan, Ouyang Gangfeng, Zhu Fang
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, Guangdong, 510275, China; SGS-CSTC Standards Technical Services Co., Ltd Guangzhou Branch, Guangzhou Economic & Technology Development, No. 198 Kezhu Road, Scientech Park, Guangzhou, Guangdong Province, 510663, China.
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, Guangdong, 510275, China.
Anal Chim Acta. 2021 Feb 8;1145:79-86. doi: 10.1016/j.aca.2020.10.063. Epub 2020 Nov 5.
Currently, nano-titanium dioxide (nTiO) is considered an emerging environmental contaminant. Bottlenecked by the traditional destructive and lethal sampling methods, nTiO's effect in living plants is poorly investigated. Here, in vivo tracing of endogenous salicylic acids at regular intervals was performed by using solid phase microextraction (SPME) technique for evaluating the effects of nTiO on plants. By planting aloe in soil containing varying amounts of nTiO, the titanium (Ti) element accumulated in the leaves to concentrations and then reached the maximum of 1.1 ± 0.4 μg/g after nTiO exceeding 0.1 g/kg. The levels of salicylic acid (SA) and acetylsalicylic acid (ASA) were up-regulated upon the exposure to nTiO, while were positively correlated to the contents of Ti. Moreover, the increased malondialdehyde, decreased total superoxide dismutase and fluctuated glutathione along with the addition of nTiO demonstrated the oxidative stress caused by nTiO. Meanwhile, apparent growth indicators including leaf elongation, plant fresh weight and root development were influenced, which further confirmed the toxicity of nTiO imparted on aloe. This study presents the possibility of using salicylic acids as biomarkers for revealing the toxicity of nTiO on plants in addition to the other biomarkers and biomass data, and the in vivo SPME technique is powerful for their monitoring.
目前，纳米二氧化钛（nTiO）被视为一种新出现的环境污染物。受传统破坏性和致死性采样方法的限制，nTiO对活体植物的影响鲜有研究。在此，通过使用固相微萃取（SPME）技术定期对植物体内的内源性水杨酸进行追踪，以评估nTiO对植物的影响。通过在含有不同量nTiO的土壤中种植芦荟，叶片中钛（Ti）元素积累至一定浓度，在nTiO超过0.1 g/kg后达到最大值1.1±0.4 μg/g。暴露于nTiO后，水杨酸（SA）和乙酰水杨酸（ASA）水平上调，且与Ti含量呈正相关。此外，随着nTiO添加量增加，丙二醛含量升高、总超氧化物歧化酶含量降低以及谷胱甘肽含量波动，表明nTiO引起了氧化应激。同时，包括叶片伸长、植物鲜重和根系发育在内的明显生长指标受到影响，这进一步证实了nTiO对芦荟的毒性。本研究表明，除了其他生物标志物和生物量数据外，水杨酸有可能作为揭示nTiO对植物毒性的生物标志物，并且体内SPME技术对其监测很有效。
