研究 CXCR3 及其配体与输卵管结核的关系。
Study on the relationship between CXCR3 and its ligands and tubal tuberculosis.
机构信息
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi 830000, China; First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, China.
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi 830000, China.
出版信息
Life Sci. 2021 May 1;272:119047. doi: 10.1016/j.lfs.2021.119047. Epub 2021 Jan 14.
OBJECTIVE
Chemokines play an important role in Mycobacterium tuberculosis infection. We aimed to investigate CXCR3, CXCL9, CXCL10 and CXCL11 to explore the correlation between the severity of tubal tuberculosis and chemokines.
METHODS
26 patients with tubal tuberculosis diagnosed in our hospital from 2016 to 2019 were selected as the experimental group, and 18 female patients who underwent high-risk pregnancy supervision in our hospital from 2016 to 2018 were selected as the control group. The pathological manifestations of tubal tuberculosis were observed by HE staining, the expressions of CXCR3 and its ligands in fallopian tubes were detected by immunohistochemistry.
RESULTS
Typical granulomatous structure of tubal tuberculosis was observed by HE staining and most of them were accompanied by massive necrosis in the experimental group, while no granulomatous lesions were found in the control group. The results of immunohistochemical staining showed that CXCR3 and its ligands were expressed in the cytoplasm and nucleus of oviduct epithelial cells and inflammatory cells, in the granuloma area. CXCL9, CXCL10 and CXCL11 were related to the severity of the disease.
KEY FINDINGS
CXCR3 and its ligands were positively expressed in tubal tuberculosis, especially CXCL9, CXCL10 and CXCL11 were positively correlated with the severity of fallopian tube disease.
SIGNIFICANCE
It is helpful for clinical diagnosis and treatment detection, and provides a new therapeutic target for the study of female genital tuberculosis in the future.
目的
趋化因子在结核分枝杆菌感染中发挥重要作用。本研究旨在探讨趋化因子受体 3（CXCR3）、趋化因子（C-X-C）配体 9（CXCL9）、趋化因子（C-X-C）配体 10（CXCL10）和趋化因子（C-X-C）配体 11 与输卵管结核严重程度的相关性。
方法
选取 2016 年至 2019 年在我院诊断为输卵管结核的 26 例患者为实验组，选取同期在我院行高危妊娠监护的 18 例女性患者为对照组。HE 染色观察输卵管结核的病理表现，免疫组织化学法检测输卵管中 CXCR3 及其配体的表达。
结果
HE 染色观察到典型的输卵管结核肉芽肿结构，实验组大部分伴有大量坏死，而对照组未见肉芽肿病变。免疫组化染色结果显示，CXCR3 及其配体在输卵管上皮细胞和炎症细胞的细胞质和细胞核中表达，在肉芽肿区域表达。CXCL9、CXCL10 和 CXCL11 与疾病严重程度相关。
主要发现
CXCR3 及其配体在输卵管结核中呈阳性表达，尤其是 CXCL9、CXCL10 和 CXCL11 与输卵管病变的严重程度呈正相关。
意义
有助于临床诊断和治疗检测，并为今后研究女性生殖器结核提供新的治疗靶点。
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